Glutathione-S-transferase protects against oxidative injury of endothelial cell tight junctions

Y. Xu, Bin Gong, Y. Yang, Y. C. Awasthi, M. Woods, P. J. Boor

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Oxidative damage of endothelial tight junction permeability is involved in the pathophysiology of a variety of vascular diseases. The authors studied the role of the antioxidant enzyme, human glutathione-S-transferase A4-4 (hGSTA4-4), in regulating expression of major molecules of tight junction in vascular endothelial cells under oxidative stress induced by H2O2. A vascular endothelial cell line, mouse pancreatic endothelial cells (MS1), was transduced with recombinant adenoviral vector containing hGSTA4-4 gene. hGSTA4-4 induced expression of tight junction proteins occludin and zonula occludens (ZO)-1 under oxidative stress. Increased hGSTA4-4 expression correlated with increased transepithelial electrical resistance and decreased tyrosine phosphorylation of occludin and ZO-1 following exposure to H2O2. In addition, morphologic dissociation of occludin, ZO-1, and F-actin during oxidative stress was reduced in hGSTA4-4-expressing cells. To explore a genetic approach for vascular diseases associated with disruption of tight junction proteins, we introduced the same viral vector to blood vessels of mice, rats, and rabbits ex vivo and found strong expression of hGSTA4-4 in endothelial cells. These results demonstrate that oxidative stress mediated disruption of tight junctions in endothelial cells may be attenuated by hGSTA4-4 expression.

Original languageEnglish (US)
Pages (from-to)333-343
Number of pages11
JournalEndothelium: Journal of Endothelial Cell Research
Volume14
Issue number6
DOIs
StatePublished - Nov 2007

Fingerprint

Intercellular Junctions
Tight Junctions
Glutathione Transferase
Endothelial Cells
Occludin
Wounds and Injuries
Oxidative Stress
Tight Junction Proteins
Vascular Diseases
leukotriene-C4 synthase
Electric Impedance
Blood Vessels
Tyrosine
Actins
Permeability
Antioxidants
Phosphorylation
Rabbits
Cell Line
Enzymes

Keywords

  • Endothelial cell
  • Glutathione-S-transferase
  • Oxidative stress
  • Tight junctions

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Glutathione-S-transferase protects against oxidative injury of endothelial cell tight junctions. / Xu, Y.; Gong, Bin; Yang, Y.; Awasthi, Y. C.; Woods, M.; Boor, P. J.

In: Endothelium: Journal of Endothelial Cell Research, Vol. 14, No. 6, 11.2007, p. 333-343.

Research output: Contribution to journalArticle

Xu, Y. ; Gong, Bin ; Yang, Y. ; Awasthi, Y. C. ; Woods, M. ; Boor, P. J. / Glutathione-S-transferase protects against oxidative injury of endothelial cell tight junctions. In: Endothelium: Journal of Endothelial Cell Research. 2007 ; Vol. 14, No. 6. pp. 333-343.
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