Glutathione s-transferases of bovine iris and ciliary body

Characterization of isoenzymes

Hassan Ahmad, Shivendra V. Singh, Satish Srivastava, Yogesh C. Awasthi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Five forms of glutathione (GSH) S-transferase (GST) having catalytic activities towards a variety of xenobiotics were present in bovine iris-ciliary body. In contrast to that in lens, cornea, and retina, GST isoenzymes belonging to all the three classes (αmu; and π were present in iris as well as in the ciliary body. GST isoenzymes of iris-ciliary body had pI values of 8.7, 7.4, 7.0, 6.6, and 6.0. GST 8.7 and GST 7.4 were apparent homodimers of 27,000 and 22,500 Mr. subunits, respectively. GST 8.7 cross-reacted only with antibodies raised against the a class GST of human liver and GST 7.4 cross-reacted with the antibodies raised against GST π of human placenta. GST 7.0 and 6.6 were heterodimers of MR 26,500 and 25,000 subunits and both these subunits cross-reacted with the antibodies raised against the μ class human GST. Iris-ciliary body contained both, GSH peroxidase I and GSH peroxidase II activities and in this respect also, they differ from lens, cornea, and retina each of which have only one of these two activities. The presence of several GST isoenzymes belonging to all the three major classes and both GSH peroxidase I and II activities in iris-ciliary body may be important for the detoxification of oxidants and xenobiotics in order to prevent their infiltration in aqueous humor.

Original languageEnglish (US)
Pages (from-to)175-184
Number of pages10
JournalCurrent Eye Research
Volume8
Issue number2
DOIs
StatePublished - 1989

Fingerprint

Ciliary Body
Iris
Transferases
Glutathione Transferase
Isoenzymes
Peroxidase
Xenobiotics
Cornea
Lenses
Retina
Antibodies
Aqueous Humor
Oxidants
Placenta

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Glutathione s-transferases of bovine iris and ciliary body : Characterization of isoenzymes. / Ahmad, Hassan; Singh, Shivendra V.; Srivastava, Satish; Awasthi, Yogesh C.

In: Current Eye Research, Vol. 8, No. 2, 1989, p. 175-184.

Research output: Contribution to journalArticle

Ahmad, Hassan ; Singh, Shivendra V. ; Srivastava, Satish ; Awasthi, Yogesh C. / Glutathione s-transferases of bovine iris and ciliary body : Characterization of isoenzymes. In: Current Eye Research. 1989 ; Vol. 8, No. 2. pp. 175-184.
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