Glycoproteomics of cerebrospinal fluid in neurodegenerative disease

Carina Sihlbom, Pia Davidsson, Mark Emmett, Alan G. Marshall, Carol L. Nilsson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cerebrospinal fluid (CSF) from individual patients with Alzheimer's disease (AD) was separated by narrow range two-dimensional (2D) gel electrophoresis and analyzed by electrospray FT-ICR MS in this glycoproteomic study. Because several altered proteins in the comparison between AD patients and healthy controls individuals are isoforms of glycoproteins, it is important to determine if the modifying glycans are also altered. FT-ICR MS and fragmentation of glycopeptides with infrared multiphoton dissociation (IRMPD) offers abundant fragment ions through breakage at the glycosidic linkages with excellent mass accuracy, which facilitates the structural determination of the site-specific N-linked glycosylation. We present results from a structural comparison of proteins from three AD patients and three control individuals of different glycosylated isomers of α-1-antitrypsin, β-trace and apolipoprotein J.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalInternational Journal of Mass Spectrometry
Volume234
Issue number1-3
DOIs
StatePublished - May 1 2004
Externally publishedYes

Fingerprint

Neurodegenerative diseases
cerebrospinal fluid
Cerebrospinal fluid
Clusterin
Glycosylation
proteins
Proteins
Glycoproteins
Glycopeptides
electrophoresis
Electrophoresis
linkages
Isomers
Polysaccharides
Protein Isoforms
fragmentation
Gels
isomers
fragments
gels

Keywords

  • Cerebrospinal fluid
  • Fourier transform
  • FT-ICR MS
  • FTMS
  • Glycoprotein
  • Ion cyclotron resonance
  • IRMPD
  • MS/MS

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy

Cite this

Glycoproteomics of cerebrospinal fluid in neurodegenerative disease. / Sihlbom, Carina; Davidsson, Pia; Emmett, Mark; Marshall, Alan G.; Nilsson, Carol L.

In: International Journal of Mass Spectrometry, Vol. 234, No. 1-3, 01.05.2004, p. 145-152.

Research output: Contribution to journalArticle

Sihlbom, Carina ; Davidsson, Pia ; Emmett, Mark ; Marshall, Alan G. ; Nilsson, Carol L. / Glycoproteomics of cerebrospinal fluid in neurodegenerative disease. In: International Journal of Mass Spectrometry. 2004 ; Vol. 234, No. 1-3. pp. 145-152.
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