Glycoproteomics of cerebrospinal fluid in neurodegenerative disease

Carina Sihlbom, Pia Davidsson, Mark R. Emmett, Alan G. Marshall, Carol L. Nilsson

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Cerebrospinal fluid (CSF) from individual patients with Alzheimer's disease (AD) was separated by narrow range two-dimensional (2D) gel electrophoresis and analyzed by electrospray FT-ICR MS in this glycoproteomic study. Because several altered proteins in the comparison between AD patients and healthy controls individuals are isoforms of glycoproteins, it is important to determine if the modifying glycans are also altered. FT-ICR MS and fragmentation of glycopeptides with infrared multiphoton dissociation (IRMPD) offers abundant fragment ions through breakage at the glycosidic linkages with excellent mass accuracy, which facilitates the structural determination of the site-specific N-linked glycosylation. We present results from a structural comparison of proteins from three AD patients and three control individuals of different glycosylated isomers of α-1-antitrypsin, β-trace and apolipoprotein J.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalInternational Journal of Mass Spectrometry
Volume234
Issue number1-3
DOIs
StatePublished - May 1 2004

Keywords

  • Cerebrospinal fluid
  • FT-ICR MS
  • FTMS
  • Fourier transform
  • Glycoprotein
  • IRMPD
  • Ion cyclotron resonance
  • MS/MS

ASJC Scopus subject areas

  • Instrumentation
  • Condensed Matter Physics
  • Spectroscopy
  • Physical and Theoretical Chemistry

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