Abstract
Patients with severe burn injuries are extremely susceptible to infection, and the host's antibacterial responses are frequently suppressed by alternatively activated macrophages (M2Mφ{symbol}), commonly demonstrated in these patients. An immunosuppressive subset of neutrophils (PMN-II), demonstrated in the peripheral blood of thermally injured patients, has been described as an inducer of M2Mφ{symbol}. In the present studies, the inhibitory effect of glycyrrhizin (GL) on M2Mφ{symbol} generation stimulated by PMN-II was examined. M2Mφ{symbol} were generated from resident Mφ{symbol} (R-Mφ{symbol}, lower chamber) after cultivation with PMN-II (upper chamber) in a dual-chamber transwell. However, M2Mφ{symbol} were not generated from R-Mφ{symbol} when the same transwell cultures were performed in the presence of GL. M2Mφ{symbol} were not generated from R-Mφ{symbol} after cultivation with PMN-II previously treated with GL, while R-Mφ{symbol} previously treated with GL converted to M2Mφ{symbol} after they were cultured with PMN-II in transwells. Interleukin-10 and CCL2 released from PMN-II were shown to be effector molecules responsible for the generation of M2Mφ{symbol}. However, these soluble factors were not produced by PMN-II treated with GL. These results indicate that GL inhibits PMN-II-stimulated M2Mφ{symbol} generation through the inhibition of CCL2/interleukin-10 production by PMN-II.
Original language | English (US) |
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Pages (from-to) | 317-322 |
Number of pages | 6 |
Journal | Cytokine |
Volume | 33 |
Issue number | 6 |
DOIs | |
State | Published - Mar 21 2006 |
Keywords
- Alternatively activated macrophages
- CCL2
- Glycyrrhizin
- Interleukin-10
- Neutrophils
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Biochemistry
- Hematology
- Molecular Biology