Glycyrrhizin inhibits neutrophil-associated generation of alternatively activated macrophages

Tsuyoshi Yoshida, Yasuhiro Tsuda, Dan Takeuchi, Makiko Kobayashi, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Patients with severe burn injuries are extremely susceptible to infection, and the host's antibacterial responses are frequently suppressed by alternatively activated macrophages (M2Mφ{symbol}), commonly demonstrated in these patients. An immunosuppressive subset of neutrophils (PMN-II), demonstrated in the peripheral blood of thermally injured patients, has been described as an inducer of M2Mφ{symbol}. In the present studies, the inhibitory effect of glycyrrhizin (GL) on M2Mφ{symbol} generation stimulated by PMN-II was examined. M2Mφ{symbol} were generated from resident Mφ{symbol} (R-Mφ{symbol}, lower chamber) after cultivation with PMN-II (upper chamber) in a dual-chamber transwell. However, M2Mφ{symbol} were not generated from R-Mφ{symbol} when the same transwell cultures were performed in the presence of GL. M2Mφ{symbol} were not generated from R-Mφ{symbol} after cultivation with PMN-II previously treated with GL, while R-Mφ{symbol} previously treated with GL converted to M2Mφ{symbol} after they were cultured with PMN-II in transwells. Interleukin-10 and CCL2 released from PMN-II were shown to be effector molecules responsible for the generation of M2Mφ{symbol}. However, these soluble factors were not produced by PMN-II treated with GL. These results indicate that GL inhibits PMN-II-stimulated M2Mφ{symbol} generation through the inhibition of CCL2/interleukin-10 production by PMN-II.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalCytokine
Volume33
Issue number6
DOIs
StatePublished - Mar 21 2006

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Keywords

  • Alternatively activated macrophages
  • CCL2
  • Glycyrrhizin
  • Interleukin-10
  • Neutrophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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