Glycyrrhizin inhibits R5 HIV replication in peripheral blood monocytes treated with 1-methyladenosine

Miwa Takei, Makiko Kobayashi, Xiao Dong Li, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

R5 HIV replicated in freshly isolated human peripheral blood monocytes after treatment with 1-methyladenosine (fresh PBM/MA), an immunosuppressive compound isolated from tumorous ascites fluids, while viral replication was not demonstrated in untreated peripheral blood monocytes (fresh PBM). The R5 HIV replication in fresh PBM/MA was inhibited by glycyrrhizin (GL). Without any other stimulation, fresh PBM/MA produced CCL2 and IL-10, while these soluble factors were not released from fresh PBM. GL greatly inhibited the production of CCL2 and IL-10 in fresh PBM/MA. After treatment with CCL2 and/or IL-10, CCR5 mRNA expression in fresh PBM was markedly enhanced, while only a trace level of the mRNA expression was detected in these cells in the absence of CCL2 or IL-10. CCR5 mRNA expression in fresh PBM treated with CCL2 or IL-10 was clearly inhibited by GL. These results indicate that GL inhibits R5 HIV replication in fresh PBM/MA through the inhibiting CCR5 expression mediated by CCL2 or IL-10.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalPathobiology
Volume72
Issue number3
DOIs
StatePublished - Apr 1 2005

Keywords

  • CCL2
  • CCR5
  • Glycyrrhizin
  • HIV

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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