Glycyrrhizin restores the impaired IL-12 production in thermally injured mice

Tokuichiro Utsunomiya, Makiko Kobayashi, Masahiko Ito, David Herndon, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mice 6 days after thermal injury (TI-mice) did not respond to lipopolysaccharide (LPS) stimulation for production of serum interleukin 12 (IL-12; 2h after LPS stimulation, <20 pg/ml in TI-mice; 1091 ± 162 pg/ml in normal mice). However, 2h after LPS stimulation, 1456 ± 118 pg/ml of IL-12 were demonstrated in sera of TI-mice previously treated with a 10 mg/kg i.p. dose of glycyrrhizin (GR). IL-12 was not induced by LPS in sera of normal mice inoculated with burn-associated type 2 T cells (IL-4/IL-10-producing CD8+ CD11b+ TCRγ/δ+ T cells isolated from spleens of TI-mice). However, IL-12 production was induced by LPS in sera of these mice previously treated with GR or a mixture of monoclonal antibodies (mAbs) for type 2 cytokines. Also, IL-12 production was induced by LPS in TI-mice inoculated with CD4+ T cells from spleens of GR-treated normal mice (GR-CD4+ T cells, 5 × 106 cells/mouse). Since GR-CD4+ T cells have been shown to be antagonistic cells against production of type 2 cytokines by burn-associated type 2 T cells, these results indicate that IL-12 unresponsiveness shown in TI-mice is recovered by GR through the regulation of burn-associated type 2 T cell responses.

Original languageEnglish (US)
Pages (from-to)49-55
Number of pages7
JournalCytokine
Volume14
Issue number1
DOIs
StatePublished - Apr 7 2001

Fingerprint

Glycyrrhizic Acid
T-cells
Interleukin-12
Lipopolysaccharides
T-Lymphocytes
Burns
Cytokines
Serum
Interleukin-4
Interleukin-10
Spleen
Monoclonal Antibodies

Keywords

  • Burn-associated type 2 T cells
  • Glycyrrhizin
  • IL-12

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Utsunomiya, T., Kobayashi, M., Ito, M., Herndon, D., Pollard, R. B., & Suzuki, F. (2001). Glycyrrhizin restores the impaired IL-12 production in thermally injured mice. Cytokine, 14(1), 49-55. https://doi.org/10.1006/cyto.2001.0847

Glycyrrhizin restores the impaired IL-12 production in thermally injured mice. / Utsunomiya, Tokuichiro; Kobayashi, Makiko; Ito, Masahiko; Herndon, David; Pollard, Richard B.; Suzuki, Fujio.

In: Cytokine, Vol. 14, No. 1, 07.04.2001, p. 49-55.

Research output: Contribution to journalArticle

Utsunomiya, T, Kobayashi, M, Ito, M, Herndon, D, Pollard, RB & Suzuki, F 2001, 'Glycyrrhizin restores the impaired IL-12 production in thermally injured mice', Cytokine, vol. 14, no. 1, pp. 49-55. https://doi.org/10.1006/cyto.2001.0847
Utsunomiya, Tokuichiro ; Kobayashi, Makiko ; Ito, Masahiko ; Herndon, David ; Pollard, Richard B. ; Suzuki, Fujio. / Glycyrrhizin restores the impaired IL-12 production in thermally injured mice. In: Cytokine. 2001 ; Vol. 14, No. 1. pp. 49-55.
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