GM-CSF enhances LTB4 release by monocytes stimulated with type III group B streptococcus (GBS)

Judy Tate, Judith L. Rowen

Research output: Contribution to journalArticle

Abstract

LTB4 and IL-8 are the predominant chemoattractants released by monocytes in response to GBS, with LTB4 predominant early (4h) and IL-8 predominant later (24h). Granulocyte-macrophage colony stimulating factor (GM-CSF) has been shown to decrease mortality in a neonatal rat model of GBS sepsis. We hypothesized that GM-CSF would enhance chemoattractant release by monocytes exposed to GBS. Adult peripheral blood monocytes were allowed to adhere in 24 well plates, 106 cells/well. Type in GBS were added (inactivated, 108 CFU/well) with or without GM-CSF. Supernatants were harvested at 4 and 24h and assayed for LTB4 by RIA and IL-8 by ELISA. IL-8 values were equivalent with or without addition of GM-CSF. LTB4 results at 4h, in pg/ml, (mean±SEM, n=4-5)are: GM-CSF, U/ml no GBS +GBS 0 124±78 423±67 25 261±78 631±47*250 262±71 656±51*2500 189±64 855±82*significant by ANOVA at 95% vs. GBS w/o GM-CSF GM-CSF significantly enhanced LTB4 release by monocytes stimulated with GBS but IL-8 release was unaffected. This suggests that LTB4 and IL-8 release are differentially regulated. We speculate that GM-CSF may be beneficial in GBS sepsis, enhancing early recruitment of neutrophils.

Original languageEnglish (US)
Pages (from-to)390
Number of pages1
JournalClinical Infectious Diseases
Volume25
Issue number2
StatePublished - 1997

Fingerprint

Streptococcus agalactiae
Leukotriene B4
Granulocyte-Macrophage Colony-Stimulating Factor
Monocytes
Interleukin-8
Chemotactic Factors
Sepsis
Neutrophil Infiltration
Streptococcus
Analysis of Variance
Enzyme-Linked Immunosorbent Assay
Mortality

ASJC Scopus subject areas

  • Immunology

Cite this

GM-CSF enhances LTB4 release by monocytes stimulated with type III group B streptococcus (GBS). / Tate, Judy; Rowen, Judith L.

In: Clinical Infectious Diseases, Vol. 25, No. 2, 1997, p. 390.

Research output: Contribution to journalArticle

@article{383437fc97f248978bdd2cf9955f40c5,
title = "GM-CSF enhances LTB4 release by monocytes stimulated with type III group B streptococcus (GBS)",
abstract = "LTB4 and IL-8 are the predominant chemoattractants released by monocytes in response to GBS, with LTB4 predominant early (4h) and IL-8 predominant later (24h). Granulocyte-macrophage colony stimulating factor (GM-CSF) has been shown to decrease mortality in a neonatal rat model of GBS sepsis. We hypothesized that GM-CSF would enhance chemoattractant release by monocytes exposed to GBS. Adult peripheral blood monocytes were allowed to adhere in 24 well plates, 106 cells/well. Type in GBS were added (inactivated, 108 CFU/well) with or without GM-CSF. Supernatants were harvested at 4 and 24h and assayed for LTB4 by RIA and IL-8 by ELISA. IL-8 values were equivalent with or without addition of GM-CSF. LTB4 results at 4h, in pg/ml, (mean±SEM, n=4-5)are: GM-CSF, U/ml no GBS +GBS 0 124±78 423±67 25 261±78 631±47*250 262±71 656±51*2500 189±64 855±82*significant by ANOVA at 95{\%} vs. GBS w/o GM-CSF GM-CSF significantly enhanced LTB4 release by monocytes stimulated with GBS but IL-8 release was unaffected. This suggests that LTB4 and IL-8 release are differentially regulated. We speculate that GM-CSF may be beneficial in GBS sepsis, enhancing early recruitment of neutrophils.",
author = "Judy Tate and Rowen, {Judith L.}",
year = "1997",
language = "English (US)",
volume = "25",
pages = "390",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - GM-CSF enhances LTB4 release by monocytes stimulated with type III group B streptococcus (GBS)

AU - Tate, Judy

AU - Rowen, Judith L.

PY - 1997

Y1 - 1997

N2 - LTB4 and IL-8 are the predominant chemoattractants released by monocytes in response to GBS, with LTB4 predominant early (4h) and IL-8 predominant later (24h). Granulocyte-macrophage colony stimulating factor (GM-CSF) has been shown to decrease mortality in a neonatal rat model of GBS sepsis. We hypothesized that GM-CSF would enhance chemoattractant release by monocytes exposed to GBS. Adult peripheral blood monocytes were allowed to adhere in 24 well plates, 106 cells/well. Type in GBS were added (inactivated, 108 CFU/well) with or without GM-CSF. Supernatants were harvested at 4 and 24h and assayed for LTB4 by RIA and IL-8 by ELISA. IL-8 values were equivalent with or without addition of GM-CSF. LTB4 results at 4h, in pg/ml, (mean±SEM, n=4-5)are: GM-CSF, U/ml no GBS +GBS 0 124±78 423±67 25 261±78 631±47*250 262±71 656±51*2500 189±64 855±82*significant by ANOVA at 95% vs. GBS w/o GM-CSF GM-CSF significantly enhanced LTB4 release by monocytes stimulated with GBS but IL-8 release was unaffected. This suggests that LTB4 and IL-8 release are differentially regulated. We speculate that GM-CSF may be beneficial in GBS sepsis, enhancing early recruitment of neutrophils.

AB - LTB4 and IL-8 are the predominant chemoattractants released by monocytes in response to GBS, with LTB4 predominant early (4h) and IL-8 predominant later (24h). Granulocyte-macrophage colony stimulating factor (GM-CSF) has been shown to decrease mortality in a neonatal rat model of GBS sepsis. We hypothesized that GM-CSF would enhance chemoattractant release by monocytes exposed to GBS. Adult peripheral blood monocytes were allowed to adhere in 24 well plates, 106 cells/well. Type in GBS were added (inactivated, 108 CFU/well) with or without GM-CSF. Supernatants were harvested at 4 and 24h and assayed for LTB4 by RIA and IL-8 by ELISA. IL-8 values were equivalent with or without addition of GM-CSF. LTB4 results at 4h, in pg/ml, (mean±SEM, n=4-5)are: GM-CSF, U/ml no GBS +GBS 0 124±78 423±67 25 261±78 631±47*250 262±71 656±51*2500 189±64 855±82*significant by ANOVA at 95% vs. GBS w/o GM-CSF GM-CSF significantly enhanced LTB4 release by monocytes stimulated with GBS but IL-8 release was unaffected. This suggests that LTB4 and IL-8 release are differentially regulated. We speculate that GM-CSF may be beneficial in GBS sepsis, enhancing early recruitment of neutrophils.

UR - http://www.scopus.com/inward/record.url?scp=33748160114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748160114&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 390

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 2

ER -