Gold-tipped elastomeric pillars for cellular mechanotransduction

S. Ghassemi, O. Rossier, Michael Sheetz, S. J. Wind, J. Hone

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The authors describe a technique for the fabrication of arrays of elastomeric pillars whose top surfaces are treated with selective chemical functionalization to promote cellular adhesion in cellular force transduction experiments. The technique involves the creation of a rigid mold consisting of arrays of circular holes into which a thin layer of Au is deposited, while the top surface of the mold and the sidewalls of the holes are protected by a sacrificial layer of Cr. When an elastomer is formed in the mold, Au adheres to the tops of the molded pillars. This can then be selectively functionalized with a protein that induces cell adhesion, while the rest of the surface is treated with a repellent substance. An additional benefit is that the tops of the pillars can be fluorescently labeled for improved accuracy in force transduction measurements.

Original languageEnglish (US)
Pages (from-to)3088-3091
Number of pages4
JournalJournal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures
Volume27
Issue number6
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

Fingerprint

Gold
gold
adhesion
Cell adhesion
elastomers
Elastomers
Adhesion
proteins
Proteins
Fabrication
fabrication
cells
Experiments

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Gold-tipped elastomeric pillars for cellular mechanotransduction. / Ghassemi, S.; Rossier, O.; Sheetz, Michael; Wind, S. J.; Hone, J.

In: Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures, Vol. 27, No. 6, 01.12.2009, p. 3088-3091.

Research output: Contribution to journalArticle

@article{dd810469e1804cef90e3329f0ecf121d,
title = "Gold-tipped elastomeric pillars for cellular mechanotransduction",
abstract = "The authors describe a technique for the fabrication of arrays of elastomeric pillars whose top surfaces are treated with selective chemical functionalization to promote cellular adhesion in cellular force transduction experiments. The technique involves the creation of a rigid mold consisting of arrays of circular holes into which a thin layer of Au is deposited, while the top surface of the mold and the sidewalls of the holes are protected by a sacrificial layer of Cr. When an elastomer is formed in the mold, Au adheres to the tops of the molded pillars. This can then be selectively functionalized with a protein that induces cell adhesion, while the rest of the surface is treated with a repellent substance. An additional benefit is that the tops of the pillars can be fluorescently labeled for improved accuracy in force transduction measurements.",
author = "S. Ghassemi and O. Rossier and Michael Sheetz and Wind, {S. J.} and J. Hone",
year = "2009",
month = "12",
day = "1",
doi = "10.1116/1.3259953",
language = "English (US)",
volume = "27",
pages = "3088--3091",
journal = "Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures",
issn = "1071-1023",
publisher = "AVS Science and Technology Society",
number = "6",

}

TY - JOUR

T1 - Gold-tipped elastomeric pillars for cellular mechanotransduction

AU - Ghassemi, S.

AU - Rossier, O.

AU - Sheetz, Michael

AU - Wind, S. J.

AU - Hone, J.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - The authors describe a technique for the fabrication of arrays of elastomeric pillars whose top surfaces are treated with selective chemical functionalization to promote cellular adhesion in cellular force transduction experiments. The technique involves the creation of a rigid mold consisting of arrays of circular holes into which a thin layer of Au is deposited, while the top surface of the mold and the sidewalls of the holes are protected by a sacrificial layer of Cr. When an elastomer is formed in the mold, Au adheres to the tops of the molded pillars. This can then be selectively functionalized with a protein that induces cell adhesion, while the rest of the surface is treated with a repellent substance. An additional benefit is that the tops of the pillars can be fluorescently labeled for improved accuracy in force transduction measurements.

AB - The authors describe a technique for the fabrication of arrays of elastomeric pillars whose top surfaces are treated with selective chemical functionalization to promote cellular adhesion in cellular force transduction experiments. The technique involves the creation of a rigid mold consisting of arrays of circular holes into which a thin layer of Au is deposited, while the top surface of the mold and the sidewalls of the holes are protected by a sacrificial layer of Cr. When an elastomer is formed in the mold, Au adheres to the tops of the molded pillars. This can then be selectively functionalized with a protein that induces cell adhesion, while the rest of the surface is treated with a repellent substance. An additional benefit is that the tops of the pillars can be fluorescently labeled for improved accuracy in force transduction measurements.

UR - http://www.scopus.com/inward/record.url?scp=72849121054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72849121054&partnerID=8YFLogxK

U2 - 10.1116/1.3259953

DO - 10.1116/1.3259953

M3 - Article

VL - 27

SP - 3088

EP - 3091

JO - Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures

JF - Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures

SN - 1071-1023

IS - 6

ER -