GPR30 is necessary for estradiol-induced desensitization of 5-HT 1A receptor signaling in the paraventricular nucleus of the rat hypothalamus

C. E. McAllister, R. D. Creech, P. A. Kimball, N. A. Muma, Q. Li

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Estrogen therapy used in combination with selective serotonin reuptake inhibitor (SSRI) treatment improves SSRI efficacy for the treatment of mood disorders. Desensitization of serotonin 1A (5-HT 1A) receptors, which takes one to two weeks to develop in animals, is necessary for SSRI therapeutic efficacy. Estradiol modifies 5-HT 1A receptor signaling and induces a partial desensitization in the paraventricular nucleus (PVN) of the rat within two days, but the mechanisms underlying this effect are currently unknown. The purpose of this study was to identify the estrogen receptor necessary for estradiol-induced 5-HT 1A receptor desensitization. We previously showed that estrogen receptor β is not necessary for 5-HT 1A receptor desensitization and that selective activation of estrogen receptor GPR30 mimics the effects of estradiol in rat PVN. Here, we used a recombinant adenovirus containing GPR30 siRNAs to decrease GPR30 expression in the PVN. Reduction of GPR30 prevented estradiol-induced desensitization of 5-HT 1A receptor as measured by hormonal responses to the selective 5-HT 1A receptor agonist, (+)8-OH-DPAT. To determine the possible mechanisms underlying these effects, we investigated protein and mRNA levels of 5-HT 1A receptor signaling components including 5-HT 1A receptor, Gαz, and RGSz1. We found that two days of estradiol increased protein and mRNA expression of RGSz1, and decreased 5-HT 1A receptor protein but increased 5-HT 1A mRNA; GPR30 knockdown prevented the estradiol-induced changes in 5-HT 1A receptor protein in the PVN. Taken together, these data demonstrate that GPR30 is necessary for estradiol-induced changes in the 5-HT 1A receptor signaling pathway and desensitization of 5-HT 1A receptor signaling.

Original languageEnglish (US)
Pages (from-to)1248-1260
Number of pages13
JournalPsychoneuroendocrinology
Volume37
Issue number8
DOIs
StatePublished - Aug 2012
Externally publishedYes

Fingerprint

Receptor, Serotonin, 5-HT1A
Paraventricular Hypothalamic Nucleus
Hypothalamus
Estradiol
Serotonin Uptake Inhibitors
Estrogen Receptors
Messenger RNA
Psychologic Desensitization
Serotonin
Proteins
8-Hydroxy-2-(di-n-propylamino)tetralin
Mood Disorders
Adenoviridae
Estrogens

Keywords

  • 5-HT receptors
  • ACTH
  • Desensitization
  • Estradiol
  • GPER
  • GPR30
  • Oxytocin
  • Paraventricular nucleus
  • RGSz1

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Psychiatry and Mental health
  • Biological Psychiatry
  • Endocrine and Autonomic Systems

Cite this

GPR30 is necessary for estradiol-induced desensitization of 5-HT 1A receptor signaling in the paraventricular nucleus of the rat hypothalamus. / McAllister, C. E.; Creech, R. D.; Kimball, P. A.; Muma, N. A.; Li, Q.

In: Psychoneuroendocrinology, Vol. 37, No. 8, 08.2012, p. 1248-1260.

Research output: Contribution to journalArticle

McAllister, C. E. ; Creech, R. D. ; Kimball, P. A. ; Muma, N. A. ; Li, Q. / GPR30 is necessary for estradiol-induced desensitization of 5-HT 1A receptor signaling in the paraventricular nucleus of the rat hypothalamus. In: Psychoneuroendocrinology. 2012 ; Vol. 37, No. 8. pp. 1248-1260.
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