Grafting of leg ulcers with undifferentiated keratinocytes

Miriam M. Brysk, Sharon S. Raimer, Rafael Pupo, Trace Bell, Srinivasan Rajaraman

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


A procedure for transplanting cultured human keratinocytes has been developed and used successfully to treat patients with persistent leg ulcers. Shave excisions of donor skin of 1 cm2 or less were expanded in culture in l week to cover wounds as large as 90 cm2. The uniqueness of this system is the use of a biochemically modified surgical dressing that permits the transplantation of undifferentiated cells. Several hours after inoculation, trypsinized single cells become attached to the dressing and are ready for grafting. Seven of eight persistent stasis ulcers completely reepithelialized after application of the modified surgical dressing with undifferentiated keratinocytes.

Original languageEnglish (US)
Pages (from-to)238-244
Number of pages7
JournalJournal of the American Academy of Dermatology
Issue number2
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Dermatology


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