Granulocyte macrophage colony-stimulating factor improves survival in two models of gut-derived sepsis by improving gut barrier function and modulating bacterial clearance

Roberto Gennari, J. Wesley Alexander, Luca Gianotti, Tonyia Eaves-Pyles, Sharon Hartmann

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52 Citations (Scopus)

Abstract

Objective: The effect of recombinant murine granulocyte macrophage colony- stimulating factor (rmGM-CSF) on survival and host defense was studied using two clinically relevant models of infection that included transfusion- induced immunosuppression. Summary Background Data: Granulocyte macrophage colony-stimulating factor improves resistance in several models of infection, but its role in transfusion-induced immunosuppression and bacterial translocation (gut-derived sepsis) has not been defined. Methods: Balb/c mice were treated with 100 ng of rmGM-CSF or placebo for 6 days in a model of transfusion, burn, and gavage, or cecal ligation and puncture (CLP). Translocation was studied in the first model. Results: Survival after transfusion, burn, and gavage was 90% in rmGM-CSF-treated animals versus 35% in the control group (p < 0.001). After CLP, survival was 75% in the rmGM- CSF group versus 30% in the control group (p = 0.01). Less translocation and better killing of bacteria was observed in the tissues in animals treated with rmGM-CSF. Conclusion: The ability of rmGM-CSF to improve gut barrier function and enhance killing of translocated organisms after burn injury induced gut origin sepsis was associated with improved outcome. Granulocyte macrophage colony-stimulating factor also improved survival after CLP.

Original languageEnglish (US)
Pages (from-to)68-76
Number of pages9
JournalAnnals of Surgery
Volume220
Issue number1
StatePublished - Jul 1994
Externally publishedYes

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Granulocyte-Macrophage Colony-Stimulating Factor
Sepsis
Punctures
Ligation
Immunosuppression
Bacterial Translocation
Control Groups
Infection
Placebos
Bacteria
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

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Granulocyte macrophage colony-stimulating factor improves survival in two models of gut-derived sepsis by improving gut barrier function and modulating bacterial clearance. / Gennari, Roberto; Alexander, J. Wesley; Gianotti, Luca; Eaves-Pyles, Tonyia; Hartmann, Sharon.

In: Annals of Surgery, Vol. 220, No. 1, 07.1994, p. 68-76.

Research output: Contribution to journalArticle

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abstract = "Objective: The effect of recombinant murine granulocyte macrophage colony- stimulating factor (rmGM-CSF) on survival and host defense was studied using two clinically relevant models of infection that included transfusion- induced immunosuppression. Summary Background Data: Granulocyte macrophage colony-stimulating factor improves resistance in several models of infection, but its role in transfusion-induced immunosuppression and bacterial translocation (gut-derived sepsis) has not been defined. Methods: Balb/c mice were treated with 100 ng of rmGM-CSF or placebo for 6 days in a model of transfusion, burn, and gavage, or cecal ligation and puncture (CLP). Translocation was studied in the first model. Results: Survival after transfusion, burn, and gavage was 90{\%} in rmGM-CSF-treated animals versus 35{\%} in the control group (p < 0.001). After CLP, survival was 75{\%} in the rmGM- CSF group versus 30{\%} in the control group (p = 0.01). Less translocation and better killing of bacteria was observed in the tissues in animals treated with rmGM-CSF. Conclusion: The ability of rmGM-CSF to improve gut barrier function and enhance killing of translocated organisms after burn injury induced gut origin sepsis was associated with improved outcome. Granulocyte macrophage colony-stimulating factor also improved survival after CLP.",
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AU - Gianotti, Luca

AU - Eaves-Pyles, Tonyia

AU - Hartmann, Sharon

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N2 - Objective: The effect of recombinant murine granulocyte macrophage colony- stimulating factor (rmGM-CSF) on survival and host defense was studied using two clinically relevant models of infection that included transfusion- induced immunosuppression. Summary Background Data: Granulocyte macrophage colony-stimulating factor improves resistance in several models of infection, but its role in transfusion-induced immunosuppression and bacterial translocation (gut-derived sepsis) has not been defined. Methods: Balb/c mice were treated with 100 ng of rmGM-CSF or placebo for 6 days in a model of transfusion, burn, and gavage, or cecal ligation and puncture (CLP). Translocation was studied in the first model. Results: Survival after transfusion, burn, and gavage was 90% in rmGM-CSF-treated animals versus 35% in the control group (p < 0.001). After CLP, survival was 75% in the rmGM- CSF group versus 30% in the control group (p = 0.01). Less translocation and better killing of bacteria was observed in the tissues in animals treated with rmGM-CSF. Conclusion: The ability of rmGM-CSF to improve gut barrier function and enhance killing of translocated organisms after burn injury induced gut origin sepsis was associated with improved outcome. Granulocyte macrophage colony-stimulating factor also improved survival after CLP.

AB - Objective: The effect of recombinant murine granulocyte macrophage colony- stimulating factor (rmGM-CSF) on survival and host defense was studied using two clinically relevant models of infection that included transfusion- induced immunosuppression. Summary Background Data: Granulocyte macrophage colony-stimulating factor improves resistance in several models of infection, but its role in transfusion-induced immunosuppression and bacterial translocation (gut-derived sepsis) has not been defined. Methods: Balb/c mice were treated with 100 ng of rmGM-CSF or placebo for 6 days in a model of transfusion, burn, and gavage, or cecal ligation and puncture (CLP). Translocation was studied in the first model. Results: Survival after transfusion, burn, and gavage was 90% in rmGM-CSF-treated animals versus 35% in the control group (p < 0.001). After CLP, survival was 75% in the rmGM- CSF group versus 30% in the control group (p = 0.01). Less translocation and better killing of bacteria was observed in the tissues in animals treated with rmGM-CSF. Conclusion: The ability of rmGM-CSF to improve gut barrier function and enhance killing of translocated organisms after burn injury induced gut origin sepsis was associated with improved outcome. Granulocyte macrophage colony-stimulating factor also improved survival after CLP.

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