Graphene quantum dots based on cannabis seeds for efficient wound healing in a mouse incisional wound model: Link with stress and neurobehavioral effect

Mohamed Amine Zarouki, Lahcen Tamegart, Lamia Hejji, Youssef Aoulad El Hadj Ali, Amina El Ayadi, Luis Pérez Villarejo, Zakaria Mennane, Badredine Souhail, Abdelmonaim Azzouz

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Graphene quantum dots (GQDs) are promising biomaterials with potential applicability in several areas due to their many useful and unique features. Among different applications, GQDs are photodynamic therapy agents that generate single oxygen and improve antimicrobial activity. In the present study, and for the first time, GQD were isolated from the Cannabis sativa L. seeds to generate C-GQDs as a new biomaterial for antibacterial and wound healing applications. Detailed characterization was performed using FTIR, UV–vis, Raman spectra, photoluminescence, TEM examination, HRTEM, ζ-potential, and XRD. Our results revealed in vitro and in vivo antibacterial activity of C-GQDs against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) with reduced minimal inhibitory concentration of 236 µg/mL for both strains. In addition, the C-GQDs confirmed the in vitro analysis and exhibited anti-inflammatory activity by reducing the level of neutrophils in blood and skin tissue. C-GQDs act by accelerating re-epithelization and granulation tissue formation. In addition, C-GQDs restored neurobehavioral alteration induced by incisional wounds by reducing oxidative stress, decreasing cortisol levels, increasing anxiolytic-like effect, and increasing vertical locomotor activity. The wound-healing effects of C-GQDs support its role as a potential therapeutic agent for diverse skin injuries.

Original languageEnglish (US)
Article number123658
JournalInternational Journal of Pharmaceutics
Volume649
DOIs
StatePublished - Jan 5 2024
Externally publishedYes

Keywords

  • Antibacterial activity
  • Anxiety
  • Cannabis seeds
  • Graphene quantum dots
  • Stress
  • Wound healing

ASJC Scopus subject areas

  • Pharmaceutical Science

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