Group B arbovirus structural and nonstructural antigens. II. Purification of Saint Louis encephalitis virus intracellular antigens

A. A. Qureshi, D. W. Trent

Research output: Contribution to journalArticle

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Abstract

Three serologically distinct antigens were identified in homogenates of Saint Louis encephalitis (SLE) virus infected cells after Brij 58 solubilization and diethylaminoethyl (DEAE) cellulose chromatography. These antigens were designated as antigen I, II, and III. Immunodiffusion analyses showed that all antigen peaks which eluted from the DEAE cellulose column contained the intracellular major envelope protein, antigen I. This protein was the only viral antigen which eluted at 0.125 M KCL in DEAE cellulose column peak C. Optical density peak D, which eluted at 0.2 M KCL, contained both antigens I and III. Antigen III was purified from this column fraction approximately 200 fold by organic solvent extraction and chromatography on hydroxylapatite and Sepharose 6B. Purified antigen III had a molecular weight of 80,000 to 85,000 and contained only one antigenic determinant as judged by immunodiffusion analysis.

Original languageEnglish (US)
Pages (from-to)985-992
Number of pages8
JournalInfection and Immunity
Volume8
Issue number6
StatePublished - 1973

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St. Louis Encephalitis Viruses
Flavivirus
Antigens
Cellulose
Immunodiffusion
Chromatography
Cetomacrogol
Viral Antigens
Durapatite
Sepharose
Epitopes
Proteins
Molecular Weight
I-antigen

ASJC Scopus subject areas

  • Immunology

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Group B arbovirus structural and nonstructural antigens. II. Purification of Saint Louis encephalitis virus intracellular antigens. / Qureshi, A. A.; Trent, D. W.

In: Infection and Immunity, Vol. 8, No. 6, 1973, p. 985-992.

Research output: Contribution to journalArticle

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