Group B arbovirus structural and nonstructural antigens. III. Serological specificity of solubilized intracellular viral proteins

A. A. Qureshi, D. W. Trent

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Solubilized nonstructural antigen III from Saint Louis encephalitis (SLE)-Japanese B encephalitis (JBE)-West Nile (WN) and dengue-2 arbovirus-infected pig kidney cells was purified by employing Brij-58 solubilization, organic solvent extraction and column chromatography. Diethylaminoethyl-cellulose column peak C eluate contained only intracellular viral envelope protein designated antigen I. Antigen I of SLE virus absorbed homologous neutralizing antibody; however, the intracellular nonstructural protein designated antigen III did not. The antigenic relationships of solubilized antigens I and III prepared from SLE, JBE, and WN virus-infected cells were determined by complement fixation (CF) and immunodiffusion analyses. Solubilized antigen I of each virus cross reacted broadly with both homologous and heterologous antibody at high antigen concentrations in the CF test. Antigen III of each virus reacted with only homologous antibody by both CF and immunoprecipitation. This study demonstrates that during SLE, JBE, WN, and dengue 2 infections, virus specific proteins containing both type specific and group reactive determinants are synthesized. Antigen III, a nonstructural protein, is serologically virus type specific and may be useful as a type specific diagnostic reagent.

Original languageEnglish (US)
Pages (from-to)993-999
Number of pages7
JournalInfection and Immunity
Volume8
Issue number6
StatePublished - 1973

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Flavivirus
Viral Proteins
St. Louis Encephalitis
Japanese Encephalitis
Antigens
Dengue
Viruses
Cetomacrogol
St. Louis Encephalitis Viruses
Viral Envelope Proteins
Heterophile Antibodies
Arboviruses
Complement Fixation Tests
West Nile virus
Proteins
Immunodiffusion
Virus Diseases
Neutralizing Antibodies
Immunoprecipitation
Cellulose

ASJC Scopus subject areas

  • Immunology

Cite this

Group B arbovirus structural and nonstructural antigens. III. Serological specificity of solubilized intracellular viral proteins. / Qureshi, A. A.; Trent, D. W.

In: Infection and Immunity, Vol. 8, No. 6, 1973, p. 993-999.

Research output: Contribution to journalArticle

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