Group B streptococcus exploits lipid rafts and phosphoinositide 3-kinase/Akt signaling pathway to invade human endometrial cells

Pawel Goluszko, Vsevolod Popov, Julie Wen, Amanda Jones, Chandra Yallampalli

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: We sought to determine the role lipid rafts and phosphoinositide 3-kinase (PI3K) in invasiveness of group B streptococci (GBS) to endometrial cells. Study Design: Antibiotic protection assay and electron microscopy were used to evaluate the invasion of GBS to human endometrial Ishikawa cells cholesterol-depleted by using methyl-beta-cyclodextrin or treated with PI3K inhibitors: wortmannin or LY294002. Immunoblotting analysis of Akt phosphorylation and cellular imaging of GFP-Akt-PH probe were used to assess PI3Ks activation in infected cells. Results: Infected Ishikawa cells streptococci are associated to membrane ruffles with morphological features of undergoing internalization. GBS remained attached but completely failed to invade to cholesterol-depleted human endometrial cells or displayed decreased invasiveness in the presence of PI3K inhibitors. Cholesterol depletion resulted in loss of membrane ruffling and dispersion of raft-associated molecules: monosialoganglioside GM1 and PI3K. Conclusion: This work provides the evidence that lipid rafts and raft-associated PI3K are implicated in GBS invasion to human endometrial cells.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume199
Issue number5
DOIs
StatePublished - Nov 2008

Fingerprint

Streptococcus agalactiae
1-Phosphatidylinositol 4-Kinase
Lipids
Cholesterol
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Membranes
Streptococcus
Phosphatidylinositol 3-Kinases
Immunoblotting
Electron Microscopy
Phosphorylation
Anti-Bacterial Agents

Keywords

  • group B streptococcus
  • Ishikawa cells
  • kinase
  • lipid rafts
  • PI3K

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Group B streptococcus exploits lipid rafts and phosphoinositide 3-kinase/Akt signaling pathway to invade human endometrial cells. / Goluszko, Pawel; Popov, Vsevolod; Wen, Julie; Jones, Amanda; Yallampalli, Chandra.

In: American Journal of Obstetrics and Gynecology, Vol. 199, No. 5, 11.2008.

Research output: Contribution to journalArticle

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AU - Popov, Vsevolod

AU - Wen, Julie

AU - Jones, Amanda

AU - Yallampalli, Chandra

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N2 - Objective: We sought to determine the role lipid rafts and phosphoinositide 3-kinase (PI3K) in invasiveness of group B streptococci (GBS) to endometrial cells. Study Design: Antibiotic protection assay and electron microscopy were used to evaluate the invasion of GBS to human endometrial Ishikawa cells cholesterol-depleted by using methyl-beta-cyclodextrin or treated with PI3K inhibitors: wortmannin or LY294002. Immunoblotting analysis of Akt phosphorylation and cellular imaging of GFP-Akt-PH probe were used to assess PI3Ks activation in infected cells. Results: Infected Ishikawa cells streptococci are associated to membrane ruffles with morphological features of undergoing internalization. GBS remained attached but completely failed to invade to cholesterol-depleted human endometrial cells or displayed decreased invasiveness in the presence of PI3K inhibitors. Cholesterol depletion resulted in loss of membrane ruffling and dispersion of raft-associated molecules: monosialoganglioside GM1 and PI3K. Conclusion: This work provides the evidence that lipid rafts and raft-associated PI3K are implicated in GBS invasion to human endometrial cells.

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