TY - JOUR
T1 - Growth characteristics of alkhumra hemorrhagic fever virus in mammalian cell lines
AU - Madani, Tariq A.
AU - Abuelzein, El Tayb M.E.
AU - Azhar, Esam I.
AU - Al-Bar, Hussein M.S.
AU - Hassan, Ahmed M.
AU - Ksiazek, Thomas G.
N1 - Publisher Copyright:
© Mary Ann Liebert, Inc.
PY - 2016/11
Y1 - 2016/11
N2 - Background: Alkhumra hemorrhagic fever virus (AHFV) is a flavivirus that was discovered in 1995 in Saudi Arabia. Clinical manifestations of AHFV infection include hemorrhagic fever, hepatitis, and encephalitis with a reported mortality rate as high as 25%. There are no published data on the growth characteristics of AHFV in mammalian cell lines. The objective of this study was to examine the ability of AHFV to grow and propagate in four of the commonly used mammalian cell culture lines and to determine the virus growth curve characteristics in each. Materials and Methods: Human epidermoid carcinoma (HEp-2), LLC-MK2, Madin-Darby canine kidney (MDCK), and Vero cell lines were inoculated with AHFV. The virus production by each cell line was determined by growth curve studies. Mean titers were calculated and expressed as median tissue culture infective dose per mL (TCID50/mL). Results: AHFV grew and propagated to variable titers in the employed cell lines. The highest mean titers were observed in the LLC-MK2, followed by the MDCK, Vero, and HEP-2, in descending order. Conclusions: The growth curve studies showed that AHFV can propagate in the four types of cell lines to variable titers. LLC-MK2 cells are superior to MDCK, Vero, and HEP-2 for propagation of AHFV.
AB - Background: Alkhumra hemorrhagic fever virus (AHFV) is a flavivirus that was discovered in 1995 in Saudi Arabia. Clinical manifestations of AHFV infection include hemorrhagic fever, hepatitis, and encephalitis with a reported mortality rate as high as 25%. There are no published data on the growth characteristics of AHFV in mammalian cell lines. The objective of this study was to examine the ability of AHFV to grow and propagate in four of the commonly used mammalian cell culture lines and to determine the virus growth curve characteristics in each. Materials and Methods: Human epidermoid carcinoma (HEp-2), LLC-MK2, Madin-Darby canine kidney (MDCK), and Vero cell lines were inoculated with AHFV. The virus production by each cell line was determined by growth curve studies. Mean titers were calculated and expressed as median tissue culture infective dose per mL (TCID50/mL). Results: AHFV grew and propagated to variable titers in the employed cell lines. The highest mean titers were observed in the LLC-MK2, followed by the MDCK, Vero, and HEP-2, in descending order. Conclusions: The growth curve studies showed that AHFV can propagate in the four types of cell lines to variable titers. LLC-MK2 cells are superior to MDCK, Vero, and HEP-2 for propagation of AHFV.
KW - Alkhumra hemorrhagic fever virus
KW - Growth curve
KW - HEp-2
KW - Indirect fluorescent antibody test
KW - LLC-MK2
KW - MDCK
KW - TCID50
KW - Vero
UR - http://www.scopus.com/inward/record.url?scp=84994589289&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84994589289&partnerID=8YFLogxK
U2 - 10.1089/vbz.2016.1968
DO - 10.1089/vbz.2016.1968
M3 - Article
C2 - 27710208
AN - SCOPUS:84994589289
SN - 1530-3667
VL - 16
SP - 722
EP - 727
JO - Vector-Borne and Zoonotic Diseases
JF - Vector-Borne and Zoonotic Diseases
IS - 11
ER -