Growth characteristics of alkhumra hemorrhagic fever virus in mammalian cell lines

Tariq A. Madani, El Tayb M E Abuelzein, Esam I. Azhar, Hussein M S Al-Bar, Ahmed M. Hassan, Thomas Ksiazek

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Alkhumra hemorrhagic fever virus (AHFV) is a flavivirus that was discovered in 1995 in Saudi Arabia. Clinical manifestations of AHFV infection include hemorrhagic fever, hepatitis, and encephalitis with a reported mortality rate as high as 25%. There are no published data on the growth characteristics of AHFV in mammalian cell lines. The objective of this study was to examine the ability of AHFV to grow and propagate in four of the commonly used mammalian cell culture lines and to determine the virus growth curve characteristics in each. Materials and Methods: Human epidermoid carcinoma (HEp-2), LLC-MK2, Madin-Darby canine kidney (MDCK), and Vero cell lines were inoculated with AHFV. The virus production by each cell line was determined by growth curve studies. Mean titers were calculated and expressed as median tissue culture infective dose per mL (TCID50/mL). Results: AHFV grew and propagated to variable titers in the employed cell lines. The highest mean titers were observed in the LLC-MK2, followed by the MDCK, Vero, and HEP-2, in descending order. Conclusions: The growth curve studies showed that AHFV can propagate in the four types of cell lines to variable titers. LLC-MK2 cells are superior to MDCK, Vero, and HEP-2 for propagation of AHFV.

Original languageEnglish (US)
Pages (from-to)722-727
Number of pages6
JournalVector-Borne and Zoonotic Diseases
Volume16
Issue number11
DOIs
StatePublished - Nov 1 2016

Fingerprint

Fever
Viruses
Cell Line
Growth
Canidae
Kidney
Flavivirus
Madin Darby Canine Kidney Cells
Vero Cells
Saudi Arabia
Virus Diseases
Encephalitis
Hepatitis
Squamous Cell Carcinoma
Cell Culture Techniques
Mortality

Keywords

  • Alkhumra hemorrhagic fever virus
  • Growth curve
  • HEp-2
  • Indirect fluorescent antibody test
  • LLC-MK2
  • MDCK
  • TCID50
  • Vero

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases
  • Virology

Cite this

Madani, T. A., Abuelzein, E. T. M. E., Azhar, E. I., Al-Bar, H. M. S., Hassan, A. M., & Ksiazek, T. (2016). Growth characteristics of alkhumra hemorrhagic fever virus in mammalian cell lines. Vector-Borne and Zoonotic Diseases, 16(11), 722-727. https://doi.org/10.1089/vbz.2016.1968

Growth characteristics of alkhumra hemorrhagic fever virus in mammalian cell lines. / Madani, Tariq A.; Abuelzein, El Tayb M E; Azhar, Esam I.; Al-Bar, Hussein M S; Hassan, Ahmed M.; Ksiazek, Thomas.

In: Vector-Borne and Zoonotic Diseases, Vol. 16, No. 11, 01.11.2016, p. 722-727.

Research output: Contribution to journalArticle

Madani, Tariq A. ; Abuelzein, El Tayb M E ; Azhar, Esam I. ; Al-Bar, Hussein M S ; Hassan, Ahmed M. ; Ksiazek, Thomas. / Growth characteristics of alkhumra hemorrhagic fever virus in mammalian cell lines. In: Vector-Borne and Zoonotic Diseases. 2016 ; Vol. 16, No. 11. pp. 722-727.
@article{84f635d00ffd48e3be53f1f0e900b2d3,
title = "Growth characteristics of alkhumra hemorrhagic fever virus in mammalian cell lines",
abstract = "Background: Alkhumra hemorrhagic fever virus (AHFV) is a flavivirus that was discovered in 1995 in Saudi Arabia. Clinical manifestations of AHFV infection include hemorrhagic fever, hepatitis, and encephalitis with a reported mortality rate as high as 25{\%}. There are no published data on the growth characteristics of AHFV in mammalian cell lines. The objective of this study was to examine the ability of AHFV to grow and propagate in four of the commonly used mammalian cell culture lines and to determine the virus growth curve characteristics in each. Materials and Methods: Human epidermoid carcinoma (HEp-2), LLC-MK2, Madin-Darby canine kidney (MDCK), and Vero cell lines were inoculated with AHFV. The virus production by each cell line was determined by growth curve studies. Mean titers were calculated and expressed as median tissue culture infective dose per mL (TCID50/mL). Results: AHFV grew and propagated to variable titers in the employed cell lines. The highest mean titers were observed in the LLC-MK2, followed by the MDCK, Vero, and HEP-2, in descending order. Conclusions: The growth curve studies showed that AHFV can propagate in the four types of cell lines to variable titers. LLC-MK2 cells are superior to MDCK, Vero, and HEP-2 for propagation of AHFV.",
keywords = "Alkhumra hemorrhagic fever virus, Growth curve, HEp-2, Indirect fluorescent antibody test, LLC-MK2, MDCK, TCID50, Vero",
author = "Madani, {Tariq A.} and Abuelzein, {El Tayb M E} and Azhar, {Esam I.} and Al-Bar, {Hussein M S} and Hassan, {Ahmed M.} and Thomas Ksiazek",
year = "2016",
month = "11",
day = "1",
doi = "10.1089/vbz.2016.1968",
language = "English (US)",
volume = "16",
pages = "722--727",
journal = "Vector-Borne and Zoonotic Diseases",
issn = "1530-3667",
publisher = "Mary Ann Liebert Inc.",
number = "11",

}

TY - JOUR

T1 - Growth characteristics of alkhumra hemorrhagic fever virus in mammalian cell lines

AU - Madani, Tariq A.

AU - Abuelzein, El Tayb M E

AU - Azhar, Esam I.

AU - Al-Bar, Hussein M S

AU - Hassan, Ahmed M.

AU - Ksiazek, Thomas

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Background: Alkhumra hemorrhagic fever virus (AHFV) is a flavivirus that was discovered in 1995 in Saudi Arabia. Clinical manifestations of AHFV infection include hemorrhagic fever, hepatitis, and encephalitis with a reported mortality rate as high as 25%. There are no published data on the growth characteristics of AHFV in mammalian cell lines. The objective of this study was to examine the ability of AHFV to grow and propagate in four of the commonly used mammalian cell culture lines and to determine the virus growth curve characteristics in each. Materials and Methods: Human epidermoid carcinoma (HEp-2), LLC-MK2, Madin-Darby canine kidney (MDCK), and Vero cell lines were inoculated with AHFV. The virus production by each cell line was determined by growth curve studies. Mean titers were calculated and expressed as median tissue culture infective dose per mL (TCID50/mL). Results: AHFV grew and propagated to variable titers in the employed cell lines. The highest mean titers were observed in the LLC-MK2, followed by the MDCK, Vero, and HEP-2, in descending order. Conclusions: The growth curve studies showed that AHFV can propagate in the four types of cell lines to variable titers. LLC-MK2 cells are superior to MDCK, Vero, and HEP-2 for propagation of AHFV.

AB - Background: Alkhumra hemorrhagic fever virus (AHFV) is a flavivirus that was discovered in 1995 in Saudi Arabia. Clinical manifestations of AHFV infection include hemorrhagic fever, hepatitis, and encephalitis with a reported mortality rate as high as 25%. There are no published data on the growth characteristics of AHFV in mammalian cell lines. The objective of this study was to examine the ability of AHFV to grow and propagate in four of the commonly used mammalian cell culture lines and to determine the virus growth curve characteristics in each. Materials and Methods: Human epidermoid carcinoma (HEp-2), LLC-MK2, Madin-Darby canine kidney (MDCK), and Vero cell lines were inoculated with AHFV. The virus production by each cell line was determined by growth curve studies. Mean titers were calculated and expressed as median tissue culture infective dose per mL (TCID50/mL). Results: AHFV grew and propagated to variable titers in the employed cell lines. The highest mean titers were observed in the LLC-MK2, followed by the MDCK, Vero, and HEP-2, in descending order. Conclusions: The growth curve studies showed that AHFV can propagate in the four types of cell lines to variable titers. LLC-MK2 cells are superior to MDCK, Vero, and HEP-2 for propagation of AHFV.

KW - Alkhumra hemorrhagic fever virus

KW - Growth curve

KW - HEp-2

KW - Indirect fluorescent antibody test

KW - LLC-MK2

KW - MDCK

KW - TCID50

KW - Vero

UR - http://www.scopus.com/inward/record.url?scp=84994589289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994589289&partnerID=8YFLogxK

U2 - 10.1089/vbz.2016.1968

DO - 10.1089/vbz.2016.1968

M3 - Article

VL - 16

SP - 722

EP - 727

JO - Vector-Borne and Zoonotic Diseases

JF - Vector-Borne and Zoonotic Diseases

SN - 1530-3667

IS - 11

ER -