Growth of mouse hepatocytes is stimulated by gastrin

Chong Zheng Yao, Richard J. Bold, Jin Ishizuka, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hepatocyte growth is regulated by various growth factors, including epidermal growth factor (EGF) and insulin. Recently, several additional peptide hormones have been shown to stimulate growth of hepatocyte only in the presence of EGF or insulin and are thus termed secondary mitogens. Gastrin regulates growth of normal and neoplastic gastrointestinal tissues, but the effect on growth of hepatocyte is unknown. We examined the effect of gastrin on growth of a normal mouse hepatocyte (NMH) line established in our laboratory. Effect of gastrin-17 (G-17) (10-8 to 10-6 M) on growth of NMH cells was examined in either the presence or absence of EGF in the culture medium. Growth of NMH cells was evaluated by incorporation of either bromodeoxyuridine (BrdU) or 3H-thymidine and by counting cells. Presence of a cell-surface receptor for G-17 was determined by Scatchard analysis using 125I-G-17. In the presence of EGF, gastrin stimulated growth of NMH cells; in the absence of EGF, gastrin did not affect growth. The stimulatory effect of gastrin on NMH cells was blocked by JMV 320, a CCK-B type receptor antagonist. NMH cells possess a single, high affinity binding site for gastrin (K(d) = 1.2 nM); EGF increased the gastrin binding capacity compared to non-treated cells (3.5 ± 0.4 vs. 2.2 ± 0.6 fmol/106 cells). G-17 stimulated growth of NMH cells through a single high affinity receptor for G-17 which pharmcologically appears to be the CCK-B type only in the presence of EGF and thus can be considered a secondary mitogen.

Original languageEnglish (US)
Pages (from-to)532-537
Number of pages6
JournalJournal of Cellular Physiology
Volume163
Issue number3
DOIs
StatePublished - Jun 1995

Fingerprint

Gastrins
Hepatocytes
Epidermal Growth Factor
Growth
Mitogens
Insulin
Cholecystokinin B Receptor
Peptide Hormones
Cell Surface Receptors
Bromodeoxyuridine
Thymidine
Culture Media
gastrin 17
Intercellular Signaling Peptides and Proteins
Binding Sites
Cells
Tissue

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Growth of mouse hepatocytes is stimulated by gastrin. / Yao, Chong Zheng; Bold, Richard J.; Ishizuka, Jin; Townsend, Courtney; Thompson, James C.

In: Journal of Cellular Physiology, Vol. 163, No. 3, 06.1995, p. 532-537.

Research output: Contribution to journalArticle

Yao, Chong Zheng ; Bold, Richard J. ; Ishizuka, Jin ; Townsend, Courtney ; Thompson, James C. / Growth of mouse hepatocytes is stimulated by gastrin. In: Journal of Cellular Physiology. 1995 ; Vol. 163, No. 3. pp. 532-537.
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