Guanosine 5'-(γ-thio) triphosphate (GTPγS) inhibits phosphorylation of insulin receptor and a novel GTP-binding protein, Gir, from human placenta

Satish Srivastava, Tushar K. Varma, Ashish C. Sinha, Ugra S. Singh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A novel 66 kDa GTP-binding protein, designated Gir, has been partially purified along with insulin receptor (IR) from human placenta. This protein binds 8-azido-GTP, is ADP-ribosylated by pertussis toxin, phosphorylated by IR tyrosine kinase and cross-reacts with antibodies against synthetic peptides from the GTP-binding domain of G (P960). Phosphorylation of IR-β subunit and Gir by IR tyrosine kinase was almost completely inhibited by 100 μM GTPγS, >75% by 50 μM and 20-30% by 1 μM, while GDP at these concentrations had no significant effect on the phosphorylation. IR tyrosine kinase phosphorylated Gir at the tyrosine residues. These studies indicate regulation of IR tyrosine kinase activity by guanosine phosphates and involvement of Gir in insulin action.

Original languageEnglish (US)
Pages (from-to)124-128
Number of pages5
JournalFEBS Letters
Volume340
Issue number1-2
DOIs
StatePublished - Feb 28 1994

Fingerprint

Phosphorylation
Guanosine
Insulin Receptor
GTP-Binding Proteins
Placenta
Guanine Nucleotides
Pertussis Toxin
Guanosine Triphosphate
Adenosine Diphosphate
Tyrosine
Insulin
Peptides
triphosphoric acid
insulin receptor tyrosine kinase
Antibodies
Proteins

Keywords

  • G-Protein
  • Insulin receptor
  • Phosphorylation
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Guanosine 5'-(γ-thio) triphosphate (GTPγS) inhibits phosphorylation of insulin receptor and a novel GTP-binding protein, Gir, from human placenta. / Srivastava, Satish; Varma, Tushar K.; Sinha, Ashish C.; Singh, Ugra S.

In: FEBS Letters, Vol. 340, No. 1-2, 28.02.1994, p. 124-128.

Research output: Contribution to journalArticle

Srivastava, Satish ; Varma, Tushar K. ; Sinha, Ashish C. ; Singh, Ugra S. / Guanosine 5'-(γ-thio) triphosphate (GTPγS) inhibits phosphorylation of insulin receptor and a novel GTP-binding protein, Gir, from human placenta. In: FEBS Letters. 1994 ; Vol. 340, No. 1-2. pp. 124-128.
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