Guidelines for sampling, measuring and reporting ionized magnesium in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing

Mohammed C. Ben Rayana, Robert W. Burnett, Arthur K. Covington, Paul D'Orazio, Niels Fogh-Andersen, Ellis Jacobs, Wolf R. Külpmann, Katsuhiko Kuwa, Lasse Larsson, Andrzej Lewenstam, Anton H.J. Maas, Gerhard Mager, Jerzy H.J. Naskalski, Anthony O. Okorodudu, Christoph Ritter, Andrew St John

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

All analyzers with ion-selective electrodes for ionized magnesium (iMg) should yield comparable and unbiased results. The prerequisite to achieve this goal is to reach consensus on sampling, measurement and reporting. The recommended guidelines for sampling, measurement and reporting iMg in plasma ("plasma" refers to circulating plasma and the forms in which it is sampled: the plasma phase of anticoagulated whole blood, plasma separated from blood cells, or serum) or blood, referring to the substance concentration of iMg in the calibrants, will provide results for iMg that are approximately 3% greater than its true concentration, and 4% less than its true molality. Binding of magnesium to proteins and ligands in plasma and blood is pH-dependent. Therefore, pH should be simultaneously measured to allow adjustment of iMg concentration to pH 7.4. The substance concentration of iMg may be physiologically and consequently clinically more relevant than the substance concentration of total magnesium.

Original languageEnglish (US)
Pages (from-to)564-569
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume43
Issue number5
DOIs
StatePublished - May 1 2005
Externally publishedYes

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Keywords

  • Erythrocyte
  • Heparin
  • Influence factors
  • Interference
  • Ion-selective electrode
  • Ionized calcium
  • Ionized magnesium
  • Lipophilic anions
  • Liquid junction potential
  • Protein
  • Sampling
  • Silicone
  • Sodium
  • pH

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Ben Rayana, M. C., Burnett, R. W., Covington, A. K., D'Orazio, P., Fogh-Andersen, N., Jacobs, E., Külpmann, W. R., Kuwa, K., Larsson, L., Lewenstam, A., Maas, A. H. J., Mager, G., Naskalski, J. H. J., Okorodudu, A. O., Ritter, C., & St John, A. (2005). Guidelines for sampling, measuring and reporting ionized magnesium in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing. Clinical Chemistry and Laboratory Medicine, 43(5), 564-569. https://doi.org/10.1515/CCLM.2005.098