Guidelines for standard preclinical experiments in the mouse model of myasthenia gravis induced by acetylcholine receptor immunization

Erdem Tuzun, Sonia Berrih-Aknin, Talma Brenner, Linda L. Kusner, Rozen Le Panse, Huan Yang, Socrates Tzartos, Premkumar Christadoss

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Myasthenia gravis (MG) is an autoimmune disorder characterized by generalized muscle weakness due to neuromuscular junction (NMJ) dysfunction brought by acetylcholine receptor (AChR) antibodies in most cases. Although steroids and other immunosuppressants are effectively used for treatment of MG, these medications often cause severe side effects and a complete remission cannot be obtained in many cases. For pre-clinical evaluation of more effective and less toxic treatment methods for MG, the experimental autoimmune myasthenia gravis (EAMG) induced by Torpedo AChR immunization has become one of the standard animal models. Although numerous compounds have been recently proposed for MG mostly by using the active immunization EAMG model, only a few have been proven to be effective in MG patients. The variability in the experimental design, immunization methods and outcome measurements of pre-clinical EAMG studies make it difficult to interpret the published reports and assess the potential for application to MG patients. In an effort to standardize the active immunization EAMG model, we propose standard procedures for animal care conditions, sampling and randomization of mice, experimental design and outcome measures. Utilization of these standard procedures might improve the power of pre-clinical EAMG experiments and increase the chances for identifying promising novel treatment methods that can be effectively translated into clinical trials for MG.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalExperimental Neurology
Volume270
DOIs
StatePublished - Oct 24 2014

Keywords

  • Experimental animal model
  • Myasthenia gravis
  • Pre-clinical experiments
  • Standard operating procedures

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Medicine(all)

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