Gut Failure and Translocation Following Burn and Sepsis

Pedro Baron, Lillian D. Traber, Daniel L. Traber, Thuan Nguyen, Maureen Hollyoak, J. P. Heggers, David Herndon

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Sepsis with multisystem organ failure is a major cause of morbidity and mortality in burns. We studied the anatomic, physiologic, and metabolic changes of gut mucosa as a normal barrier against sepsis and systemic inflammatory response after burn and sepsis in the chronic porcine model. Flow probes were placed on the mesenteric and hepatic arteries and portal vein. Catheters were placed in the pulmonary artery (Swan-Ganz), aorta, superior mesenteric, and hepatic veins. After 5 days, baseline data were collected and studied after a 40%, third degree burn. They were resuscitated with Ringer's lactate solution (Parkland formula). Eighteen hours later, Escherichia coli endotoxin (100 μg/kg) was administered. All animals were sacrificed after 30 hr. The data were compared to a group of sham animals. Following thermal injury the cardiovascular status was stable. Endotoxin administration decreased systemic vascular resistance index and mean arterial pressure, but increased cardiac index. Mesenteric blood flow, vascular resistance, and oxygen consumption showed a transient fall after endotoxin infusion with 20, 23, and 40% reduction, respectively. These changes were associated with a rise in plasma levels of conjugated dienes. The intestinal ornithine decarboxylase activity was elevated at the end of the experiment, evidence of gut repair. Gut bacteria translocated into mesenteric lymph nodes, spleen, and burn wounds in 50% of the animals. We concluded that bacterial translocation into mesenteric lymph nodes, spleen, and wound is due to gut mucosal failure after burn trauma and sepsis. These pathophysiologic changes may be the result of mesenteric ischemia and reperfusion injury.

Original languageEnglish (US)
Pages (from-to)197-204
Number of pages8
JournalJournal of Surgical Research
Volume57
Issue number1
DOIs
StatePublished - Jul 1994

Fingerprint

Sepsis
Hepatic Veins
Wounds and Injuries
Endotoxins
Vascular Resistance
Spleen
Lymph Nodes
Bacterial Translocation
Mesenteric Veins
Mesenteric Arteries
Ornithine Decarboxylase
Hepatic Artery
Portal Vein
Reperfusion Injury
Burns
Oxygen Consumption
Pulmonary Artery
Aorta
Arterial Pressure
Mucous Membrane

ASJC Scopus subject areas

  • Surgery

Cite this

Baron, P., Traber, L. D., Traber, D. L., Nguyen, T., Hollyoak, M., Heggers, J. P., & Herndon, D. (1994). Gut Failure and Translocation Following Burn and Sepsis. Journal of Surgical Research, 57(1), 197-204. https://doi.org/10.1006/jsre.1994.1131

Gut Failure and Translocation Following Burn and Sepsis. / Baron, Pedro; Traber, Lillian D.; Traber, Daniel L.; Nguyen, Thuan; Hollyoak, Maureen; Heggers, J. P.; Herndon, David.

In: Journal of Surgical Research, Vol. 57, No. 1, 07.1994, p. 197-204.

Research output: Contribution to journalArticle

Baron, P, Traber, LD, Traber, DL, Nguyen, T, Hollyoak, M, Heggers, JP & Herndon, D 1994, 'Gut Failure and Translocation Following Burn and Sepsis', Journal of Surgical Research, vol. 57, no. 1, pp. 197-204. https://doi.org/10.1006/jsre.1994.1131
Baron P, Traber LD, Traber DL, Nguyen T, Hollyoak M, Heggers JP et al. Gut Failure and Translocation Following Burn and Sepsis. Journal of Surgical Research. 1994 Jul;57(1):197-204. https://doi.org/10.1006/jsre.1994.1131
Baron, Pedro ; Traber, Lillian D. ; Traber, Daniel L. ; Nguyen, Thuan ; Hollyoak, Maureen ; Heggers, J. P. ; Herndon, David. / Gut Failure and Translocation Following Burn and Sepsis. In: Journal of Surgical Research. 1994 ; Vol. 57, No. 1. pp. 197-204.
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