H-NS Regulates Gene Expression and Compacts the Nucleoid: Insights from Single-Molecule Experiments

Ricksen S. Winardhi, Jie Yan, Linda Kenney

Research output: Contribution to journalReview article

36 Citations (Scopus)

Abstract

A set of abundant nucleoid-associated proteins (NAPs) play key functions in organizing the bacterial chromosome and regulating gene transcription globally. Histone-like nucleoid structuring protein (H-NS) is representative of a family of NAPs that are widespread across bacterial species. They have drawn extensive attention due to their crucial function in gene silencing in bacterial pathogens. Recent rapid progress in single-molecule manipulation and imaging technologies has made it possible to directly probe DNA binding by H-NS, its impact on DNA conformation and topology, and its competition with other DNA-binding proteins at the single-DNA-molecule level. Here, we review recent findings from such studies, and provide our views on how these findings yield new insights into the understanding of the roles of H-NS family members in DNA organization and gene silencing.

Original languageEnglish (US)
Pages (from-to)1321-1329
Number of pages9
JournalBiophysical Journal
Volume109
Issue number7
DOIs
StatePublished - Oct 6 2015
Externally publishedYes

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Histones
Gene Expression
Gene Silencing
Proteins
Bacterial Chromosomes
Nucleic Acid Conformation
DNA
DNA Probes
DNA-Binding Proteins
Organizations
Technology
Genes

ASJC Scopus subject areas

  • Biophysics

Cite this

H-NS Regulates Gene Expression and Compacts the Nucleoid : Insights from Single-Molecule Experiments. / Winardhi, Ricksen S.; Yan, Jie; Kenney, Linda.

In: Biophysical Journal, Vol. 109, No. 7, 06.10.2015, p. 1321-1329.

Research output: Contribution to journalReview article

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