Haemorrhagic fever with renal syndrome

evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM

B. Niklasson, E. Tkachenko, A. P. Ivanov, G. van der Groen, D. Wiger, H. K. Andersen, James LeDuc, T. Kjelsson, K. Nyström

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR. IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies. Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the "one-way" crossing described earlier.

Original languageEnglish (US)
Pages (from-to)637-648
Number of pages12
JournalResearch in Virology
Volume141
Issue number6
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Puumala virus
Hemorrhagic Fever with Renal Syndrome
Immunoglobulin M
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Serum
USSR
Belgium
Seroepidemiologic Studies
Denmark
Norway
Korea
Sweden
Reading
Immunity
Antibodies

Keywords

  • Belgium
  • Bunyaviridae
  • Diagnosis
  • ELISA
  • Haemorrhagic fever
  • IgG
  • IgM
  • Korea
  • Puumala virus
  • Renal syndrome
  • Scandinavia
  • USSR

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Niklasson, B., Tkachenko, E., Ivanov, A. P., van der Groen, G., Wiger, D., Andersen, H. K., ... Nyström, K. (1990). Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM. Research in Virology, 141(6), 637-648. https://doi.org/10.1016/0923-2516(90)90036-I

Haemorrhagic fever with renal syndrome : evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM. / Niklasson, B.; Tkachenko, E.; Ivanov, A. P.; van der Groen, G.; Wiger, D.; Andersen, H. K.; LeDuc, James; Kjelsson, T.; Nyström, K.

In: Research in Virology, Vol. 141, No. 6, 1990, p. 637-648.

Research output: Contribution to journalArticle

Niklasson, B, Tkachenko, E, Ivanov, AP, van der Groen, G, Wiger, D, Andersen, HK, LeDuc, J, Kjelsson, T & Nyström, K 1990, 'Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM', Research in Virology, vol. 141, no. 6, pp. 637-648. https://doi.org/10.1016/0923-2516(90)90036-I
Niklasson, B. ; Tkachenko, E. ; Ivanov, A. P. ; van der Groen, G. ; Wiger, D. ; Andersen, H. K. ; LeDuc, James ; Kjelsson, T. ; Nyström, K. / Haemorrhagic fever with renal syndrome : evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM. In: Research in Virology. 1990 ; Vol. 141, No. 6. pp. 637-648.
@article{7c9f4a3999e24b54a30d29124ab2ae2b,
title = "Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM",
abstract = "IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR. IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies. Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the {"}one-way{"} crossing described earlier.",
keywords = "Belgium, Bunyaviridae, Diagnosis, ELISA, Haemorrhagic fever, IgG, IgM, Korea, Puumala virus, Renal syndrome, Scandinavia, USSR",
author = "B. Niklasson and E. Tkachenko and Ivanov, {A. P.} and {van der Groen}, G. and D. Wiger and Andersen, {H. K.} and James LeDuc and T. Kjelsson and K. Nystr{\"o}m",
year = "1990",
doi = "10.1016/0923-2516(90)90036-I",
language = "English (US)",
volume = "141",
pages = "637--648",
journal = "Research in Virology",
issn = "0923-2516",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - Haemorrhagic fever with renal syndrome

T2 - evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM

AU - Niklasson, B.

AU - Tkachenko, E.

AU - Ivanov, A. P.

AU - van der Groen, G.

AU - Wiger, D.

AU - Andersen, H. K.

AU - LeDuc, James

AU - Kjelsson, T.

AU - Nyström, K.

PY - 1990

Y1 - 1990

N2 - IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR. IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies. Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the "one-way" crossing described earlier.

AB - IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR. IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies. Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the "one-way" crossing described earlier.

KW - Belgium

KW - Bunyaviridae

KW - Diagnosis

KW - ELISA

KW - Haemorrhagic fever

KW - IgG

KW - IgM

KW - Korea

KW - Puumala virus

KW - Renal syndrome

KW - Scandinavia

KW - USSR

UR - http://www.scopus.com/inward/record.url?scp=0025638921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025638921&partnerID=8YFLogxK

U2 - 10.1016/0923-2516(90)90036-I

DO - 10.1016/0923-2516(90)90036-I

M3 - Article

VL - 141

SP - 637

EP - 648

JO - Research in Virology

JF - Research in Virology

SN - 0923-2516

IS - 6

ER -