Abstract
The transfection of subconfluent monolayers of HEL cells with Xba I endonuclease fragmented HCMV-DNA resulted in a large number of morphologically transformed foci. The frequency of focus formation of Xba I fragmented DNA increased after TPA treatment of the cells. The morphologically altered cells showed specific reactivity with anti-HCMV serum pool in the nuclear and perinuclear region and in the cytoplasm. The HCMV-32P-labelled probe DNA has been specifically hybridized to the cells of morphologically altered foci. The autoradiographic grains were localized in both nucleic and cytoplasm of the cells showing a specific hybridization between the probe DNA and nuclear as well as polyribosomal RNA.
Original language | English (US) |
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Pages (from-to) | 167-173 |
Number of pages | 7 |
Journal | Acta microbiologica Hungarica |
Volume | 32 |
Issue number | 2 |
State | Published - 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)