HCMV specific expression in HEL cells transformed by Xba I endonuclease fragmented HCMV-DNA.

Istvan Boldogh, E. S. Huang, J. F. Baskar, L. Váczi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The transfection of subconfluent monolayers of HEL cells with Xba I endonuclease fragmented HCMV-DNA resulted in a large number of morphologically transformed foci. The frequency of focus formation of Xba I fragmented DNA increased after TPA treatment of the cells. The morphologically altered cells showed specific reactivity with anti-HCMV serum pool in the nuclear and perinuclear region and in the cytoplasm. The HCMV-32P-labelled probe DNA has been specifically hybridized to the cells of morphologically altered foci. The autoradiographic grains were localized in both nucleic and cytoplasm of the cells showing a specific hybridization between the probe DNA and nuclear as well as polyribosomal RNA.

Original languageEnglish (US)
Pages (from-to)167-173
Number of pages7
JournalActa Microbiologica Hungarica
Volume32
Issue number2
StatePublished - 1985
Externally publishedYes

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Deoxyribonuclease I
DNA
DNA Probes
Cytoplasm
Transfection
RNA
Serum

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

HCMV specific expression in HEL cells transformed by Xba I endonuclease fragmented HCMV-DNA. / Boldogh, Istvan; Huang, E. S.; Baskar, J. F.; Váczi, L.

In: Acta Microbiologica Hungarica, Vol. 32, No. 2, 1985, p. 167-173.

Research output: Contribution to journalArticle

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