TY - JOUR
T1 - Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model
AU - Ito, Hiroshi
AU - Asmussen, Sven
AU - Traber, Daniel L.
AU - Cox, Robert A.
AU - Hawkins, Hal K.
AU - Connelly, Rhykka
AU - Traber, Lillian D.
AU - Walker, Timothy W.
AU - Malgerud, Erik
AU - Sakurai, Hiroyuki
AU - Enkhbaatar, Perenlei
N1 - Funding Information:
The authors thank the staff of the Investigational Intensive Care Unit at the University of Texas Medical Branch for their valuable assistance in conducting these studies. This study was supported by grants from the Shriner's of North America , #85500 , #85410 .
PY - 2014/5
Y1 - 2014/5
N2 - To investigate the efficacy of sea buckthorn (SBT) seed oil - a rich source of substances known to have anti-atherogenic and cardioprotective activity, and to promote skin and mucosa epithelization - on burn wound healing, five adult sheep were subjected to 3rd degree flame burns. Two burn sites were made on the dorsum of the sheep and the eschar was excised down to the fascia. Split-thickness skin grafts were harvested, meshed, and fitted to the wounds. The autograft was placed on the fascia and SBT seed oil was topically applied to one recipient and one donor site, respectively, with the remaining sites treated with vehicle. The wound blood flow (LASER Doppler), and epithelization (ultrasound) were determined at 6, 14, and 21 days after injury. 14 days after grafting, the percentage of epithelization in the treated sites was greater (95 ± 2.2% vs. 83 ± 2.9%, p < 0.05) than in the untreated sites. Complete epithelization time was shorter in both treated recipient and donor sites (14.20 ± 0.48 vs. 19.60 ± 0.40 days, p < 0.05 and 13.40 ± 1.02 vs. 19.60 ± 0.50 days, p < 0.05, respectively) than in the untreated sites, confirmed by ultrasound. In conclusion, SBT seed oil has significant wound healing activity in full-thickness burns and split-thickness harvested wounds.
AB - To investigate the efficacy of sea buckthorn (SBT) seed oil - a rich source of substances known to have anti-atherogenic and cardioprotective activity, and to promote skin and mucosa epithelization - on burn wound healing, five adult sheep were subjected to 3rd degree flame burns. Two burn sites were made on the dorsum of the sheep and the eschar was excised down to the fascia. Split-thickness skin grafts were harvested, meshed, and fitted to the wounds. The autograft was placed on the fascia and SBT seed oil was topically applied to one recipient and one donor site, respectively, with the remaining sites treated with vehicle. The wound blood flow (LASER Doppler), and epithelization (ultrasound) were determined at 6, 14, and 21 days after injury. 14 days after grafting, the percentage of epithelization in the treated sites was greater (95 ± 2.2% vs. 83 ± 2.9%, p < 0.05) than in the untreated sites. Complete epithelization time was shorter in both treated recipient and donor sites (14.20 ± 0.48 vs. 19.60 ± 0.40 days, p < 0.05 and 13.40 ± 1.02 vs. 19.60 ± 0.50 days, p < 0.05, respectively) than in the untreated sites, confirmed by ultrasound. In conclusion, SBT seed oil has significant wound healing activity in full-thickness burns and split-thickness harvested wounds.
KW - Burn wounds
KW - Sea buckthorn seed oil
KW - Skin autograft
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U2 - 10.1016/j.burns.2013.08.011
DO - 10.1016/j.burns.2013.08.011
M3 - Article
C2 - 24007892
AN - SCOPUS:84896072287
SN - 0305-4179
VL - 40
SP - 511
EP - 519
JO - Burns
JF - Burns
IS - 3
ER -