Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model

Hiroshi Ito, Sven Asmussen, Daniel L. Traber, Robert A. Cox, Hal K. Hawkins, Rhykka Connelly, Lillian D. Traber, Timothy W. Walker, Erik Malgerud, Hiroyuki Sakurai, Perenlei Enkhbaatar

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To investigate the efficacy of sea buckthorn (SBT) seed oil - a rich source of substances known to have anti-atherogenic and cardioprotective activity, and to promote skin and mucosa epithelization - on burn wound healing, five adult sheep were subjected to 3rd degree flame burns. Two burn sites were made on the dorsum of the sheep and the eschar was excised down to the fascia. Split-thickness skin grafts were harvested, meshed, and fitted to the wounds. The autograft was placed on the fascia and SBT seed oil was topically applied to one recipient and one donor site, respectively, with the remaining sites treated with vehicle. The wound blood flow (LASER Doppler), and epithelization (ultrasound) were determined at 6, 14, and 21 days after injury. 14 days after grafting, the percentage of epithelization in the treated sites was greater (95 ± 2.2% vs. 83 ± 2.9%, p < 0.05) than in the untreated sites. Complete epithelization time was shorter in both treated recipient and donor sites (14.20 ± 0.48 vs. 19.60 ± 0.40 days, p < 0.05 and 13.40 ± 1.02 vs. 19.60 ± 0.50 days, p < 0.05, respectively) than in the untreated sites, confirmed by ultrasound. In conclusion, SBT seed oil has significant wound healing activity in full-thickness burns and split-thickness harvested wounds.

Original languageEnglish (US)
Pages (from-to)511-519
Number of pages9
JournalBurns
Volume40
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Hippophae
Sheep
Seeds
Oils
Fascia
Wounds and Injuries
Burns
Wound Healing
Doppler Ultrasonography
Skin
Autografts
Mucous Membrane
Transplants

Keywords

  • Burn wounds
  • Sea buckthorn seed oil
  • Skin autograft

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine
  • Surgery

Cite this

Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model. / Ito, Hiroshi; Asmussen, Sven; Traber, Daniel L.; Cox, Robert A.; Hawkins, Hal K.; Connelly, Rhykka; Traber, Lillian D.; Walker, Timothy W.; Malgerud, Erik; Sakurai, Hiroyuki; Enkhbaatar, Perenlei.

In: Burns, Vol. 40, No. 3, 2014, p. 511-519.

Research output: Contribution to journalArticle

Ito, H, Asmussen, S, Traber, DL, Cox, RA, Hawkins, HK, Connelly, R, Traber, LD, Walker, TW, Malgerud, E, Sakurai, H & Enkhbaatar, P 2014, 'Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model', Burns, vol. 40, no. 3, pp. 511-519. https://doi.org/10.1016/j.burns.2013.08.011
Ito, Hiroshi ; Asmussen, Sven ; Traber, Daniel L. ; Cox, Robert A. ; Hawkins, Hal K. ; Connelly, Rhykka ; Traber, Lillian D. ; Walker, Timothy W. ; Malgerud, Erik ; Sakurai, Hiroyuki ; Enkhbaatar, Perenlei. / Healing efficacy of sea buckthorn (Hippophae rhamnoides L.) seed oil in an ovine burn wound model. In: Burns. 2014 ; Vol. 40, No. 3. pp. 511-519.
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AB - To investigate the efficacy of sea buckthorn (SBT) seed oil - a rich source of substances known to have anti-atherogenic and cardioprotective activity, and to promote skin and mucosa epithelization - on burn wound healing, five adult sheep were subjected to 3rd degree flame burns. Two burn sites were made on the dorsum of the sheep and the eschar was excised down to the fascia. Split-thickness skin grafts were harvested, meshed, and fitted to the wounds. The autograft was placed on the fascia and SBT seed oil was topically applied to one recipient and one donor site, respectively, with the remaining sites treated with vehicle. The wound blood flow (LASER Doppler), and epithelization (ultrasound) were determined at 6, 14, and 21 days after injury. 14 days after grafting, the percentage of epithelization in the treated sites was greater (95 ± 2.2% vs. 83 ± 2.9%, p < 0.05) than in the untreated sites. Complete epithelization time was shorter in both treated recipient and donor sites (14.20 ± 0.48 vs. 19.60 ± 0.40 days, p < 0.05 and 13.40 ± 1.02 vs. 19.60 ± 0.50 days, p < 0.05, respectively) than in the untreated sites, confirmed by ultrasound. In conclusion, SBT seed oil has significant wound healing activity in full-thickness burns and split-thickness harvested wounds.

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