Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells

Sayaka Higuchi, Qingsong Lin, Jigang Wang, Teck Kwang Lim, Shashikant B. Joshi, Ganesh Srinivasan Anand, Maxey C M Chung, Michael Sheetz, Hideaki Fujita

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.

Original languageEnglish (US)
Pages (from-to)320-325
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume115
Issue number3
DOIs
StatePublished - Mar 1 2013
Externally publishedYes

Fingerprint

Stem cells
Cardiac Myocytes
Extracellular Matrix
Tissue
Liver
Cardiac Myosins
Troponin I
Myosin Heavy Chains
Proteins
Embryonic Stem Cells
Cell proliferation
Cell death
Mouse Embryonic Stem Cells
Elasticity
Extracellular Matrix Proteins
Specifications
Cell Lineage
Proteomics
Cell Differentiation
Cell Death

Keywords

  • Cardiomyocyte
  • Differentiation
  • Embryonic stem cells
  • Extracellular matrix
  • Mechanobiology

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells. / Higuchi, Sayaka; Lin, Qingsong; Wang, Jigang; Lim, Teck Kwang; Joshi, Shashikant B.; Anand, Ganesh Srinivasan; Chung, Maxey C M; Sheetz, Michael; Fujita, Hideaki.

In: Journal of Bioscience and Bioengineering, Vol. 115, No. 3, 01.03.2013, p. 320-325.

Research output: Contribution to journalArticle

Higuchi, Sayaka ; Lin, Qingsong ; Wang, Jigang ; Lim, Teck Kwang ; Joshi, Shashikant B. ; Anand, Ganesh Srinivasan ; Chung, Maxey C M ; Sheetz, Michael ; Fujita, Hideaki. / Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells. In: Journal of Bioscience and Bioengineering. 2013 ; Vol. 115, No. 3. pp. 320-325.
@article{7f73716ffd2048c99d886ab317bbc2fe,
title = "Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells",
abstract = "We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.",
keywords = "Cardiomyocyte, Differentiation, Embryonic stem cells, Extracellular matrix, Mechanobiology",
author = "Sayaka Higuchi and Qingsong Lin and Jigang Wang and Lim, {Teck Kwang} and Joshi, {Shashikant B.} and Anand, {Ganesh Srinivasan} and Chung, {Maxey C M} and Michael Sheetz and Hideaki Fujita",
year = "2013",
month = "3",
day = "1",
doi = "10.1016/j.jbiosc.2012.10.004",
language = "English (US)",
volume = "115",
pages = "320--325",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells

AU - Higuchi, Sayaka

AU - Lin, Qingsong

AU - Wang, Jigang

AU - Lim, Teck Kwang

AU - Joshi, Shashikant B.

AU - Anand, Ganesh Srinivasan

AU - Chung, Maxey C M

AU - Sheetz, Michael

AU - Fujita, Hideaki

PY - 2013/3/1

Y1 - 2013/3/1

N2 - We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.

AB - We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.

KW - Cardiomyocyte

KW - Differentiation

KW - Embryonic stem cells

KW - Extracellular matrix

KW - Mechanobiology

UR - http://www.scopus.com/inward/record.url?scp=84872333448&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872333448&partnerID=8YFLogxK

U2 - 10.1016/j.jbiosc.2012.10.004

DO - 10.1016/j.jbiosc.2012.10.004

M3 - Article

C2 - 23168383

AN - SCOPUS:84872333448

VL - 115

SP - 320

EP - 325

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 3

ER -