Heat-shock induces abnormalities in the cellular distribution of amyloid precursor protein (APP) and APP fusion proteins

Miguel Pappolla, K. Sambamurti, S. Efthimiopoulos, L. Refolo, R. A. Omar, N. K. Robakis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The heat shock or stress response may play a role in the pathogenesis of Alzheimer's disease. We conducted experiments to visualize microscopically the distribution of wild type amyloid precursor protein (APP) and the behavior of an APP deletion mutant under stress. This was achieved by heat-shock treatment of cells expressing fusion recombinant APP proteins tagged with secreted placental alkaline phosphatase (SEAP). The fusion proteins were cleaved and secreted in a manner similar to wild type APP in unstressed control cells. SEAP activity was detected by cytochemical methods within the cytoplasm in less than 10% of transfected unstressed cells. Heat shocked cells showed a striking difference from the control cells in that over 90% of the stressed cells displayed strong intracytoplasmic SEAP activity occurring with Golgi-like pattern and/or membranous distribution. The effects of heat shock were not due to a peculiar behavior of the clones and depended on the APP portion of the constructs. This study shows miscompartmentalization of APP under stress. Such cellular changes may bear important implications in the processing of APP.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalNeuroscience Letters
Volume192
Issue number2
DOIs
StatePublished - Jun 9 1995
Externally publishedYes

Fingerprint

Amyloid beta-Protein Precursor
Shock
Hot Temperature
Heat-Shock Response
Proteins
Cell Fusion
Recombinant Proteins
Amyloid
Alzheimer Disease
Cytoplasm
Clone Cells
placental alkaline phosphatase

Keywords

  • Alzheimer's disease
  • Amyloid precursor protein
  • Heat shock proteins
  • Placental alkaline phosphatase
  • Stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Heat-shock induces abnormalities in the cellular distribution of amyloid precursor protein (APP) and APP fusion proteins. / Pappolla, Miguel; Sambamurti, K.; Efthimiopoulos, S.; Refolo, L.; Omar, R. A.; Robakis, N. K.

In: Neuroscience Letters, Vol. 192, No. 2, 09.06.1995, p. 105-108.

Research output: Contribution to journalArticle

Pappolla, Miguel ; Sambamurti, K. ; Efthimiopoulos, S. ; Refolo, L. ; Omar, R. A. ; Robakis, N. K. / Heat-shock induces abnormalities in the cellular distribution of amyloid precursor protein (APP) and APP fusion proteins. In: Neuroscience Letters. 1995 ; Vol. 192, No. 2. pp. 105-108.
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