Heat shock inhibits activation of NF-κB in the absence of heat shock factor-1

Vivek Malhotra, Tonyia Eaves-Pyles, Kelli Odoms, Gina Quaid, Thomas P. Shanley, Hector R. Wong

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The heat shock response is known to inhibit NF-κB activation and NF-κB-dependent gene expression. Herein we determined if cells lacking heat shock factor-1 (HSF-1), the major transcription factor regulating heat shock protein gene expression, have an altered ability to modulate NF-κB activation. Embryonic fibroblasts from HSF-1-null mutant mice (HSF-1-/- cells) had a drastically reduced ability to express heat shock protein-70 in response to heat shock, compared to embryonic fibroblasts from wild-type mice (HSF+/+ cells). There was no difference, however, in the ability of heat shock to inhibit TNFα-mediated NF-κB activation, IκBα degradation, IκB kinase activation, and macrophage chemotactic protein-1 expression in the HSF-1-/- cells compared to the HSF-1+/+ cells. These data demonstrate that heat shock inhibits activation of the NF-κB/IκBα pathway and NF-κB-dependent gene expression in the absence of an intact heat shock response.

Original languageEnglish (US)
Pages (from-to)453-457
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Shock
Hot Temperature
Chemical activation
Heat-Shock Response
Gene Expression
Gene expression
Fibroblasts
HSP70 Heat-Shock Proteins
Macrophage Activation
Heat-Shock Proteins
Transcription Factors
Phosphotransferases
Macrophages
Degradation
Proteins

Keywords

  • Cellular activation
  • Costimulation
  • Heat shock
  • Heat shock factor
  • Heat shock proteins
  • Iκ kinase
  • IκBα
  • NF-κB
  • Transcription factors

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Heat shock inhibits activation of NF-κB in the absence of heat shock factor-1. / Malhotra, Vivek; Eaves-Pyles, Tonyia; Odoms, Kelli; Quaid, Gina; Shanley, Thomas P.; Wong, Hector R.

In: Biochemical and Biophysical Research Communications, Vol. 291, No. 3, 2002, p. 453-457.

Research output: Contribution to journalArticle

Malhotra, Vivek ; Eaves-Pyles, Tonyia ; Odoms, Kelli ; Quaid, Gina ; Shanley, Thomas P. ; Wong, Hector R. / Heat shock inhibits activation of NF-κB in the absence of heat shock factor-1. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 291, No. 3. pp. 453-457.
@article{46eed6e63f8a4c59aae145a60e5fb65d,
title = "Heat shock inhibits activation of NF-κB in the absence of heat shock factor-1",
abstract = "The heat shock response is known to inhibit NF-κB activation and NF-κB-dependent gene expression. Herein we determined if cells lacking heat shock factor-1 (HSF-1), the major transcription factor regulating heat shock protein gene expression, have an altered ability to modulate NF-κB activation. Embryonic fibroblasts from HSF-1-null mutant mice (HSF-1-/- cells) had a drastically reduced ability to express heat shock protein-70 in response to heat shock, compared to embryonic fibroblasts from wild-type mice (HSF+/+ cells). There was no difference, however, in the ability of heat shock to inhibit TNFα-mediated NF-κB activation, IκBα degradation, IκB kinase activation, and macrophage chemotactic protein-1 expression in the HSF-1-/- cells compared to the HSF-1+/+ cells. These data demonstrate that heat shock inhibits activation of the NF-κB/IκBα pathway and NF-κB-dependent gene expression in the absence of an intact heat shock response.",
keywords = "Cellular activation, Costimulation, Heat shock, Heat shock factor, Heat shock proteins, Iκ kinase, IκBα, NF-κB, Transcription factors",
author = "Vivek Malhotra and Tonyia Eaves-Pyles and Kelli Odoms and Gina Quaid and Shanley, {Thomas P.} and Wong, {Hector R.}",
year = "2002",
doi = "10.1006/bbrc.2002.6470",
language = "English (US)",
volume = "291",
pages = "453--457",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Heat shock inhibits activation of NF-κB in the absence of heat shock factor-1

AU - Malhotra, Vivek

AU - Eaves-Pyles, Tonyia

AU - Odoms, Kelli

AU - Quaid, Gina

AU - Shanley, Thomas P.

AU - Wong, Hector R.

PY - 2002

Y1 - 2002

N2 - The heat shock response is known to inhibit NF-κB activation and NF-κB-dependent gene expression. Herein we determined if cells lacking heat shock factor-1 (HSF-1), the major transcription factor regulating heat shock protein gene expression, have an altered ability to modulate NF-κB activation. Embryonic fibroblasts from HSF-1-null mutant mice (HSF-1-/- cells) had a drastically reduced ability to express heat shock protein-70 in response to heat shock, compared to embryonic fibroblasts from wild-type mice (HSF+/+ cells). There was no difference, however, in the ability of heat shock to inhibit TNFα-mediated NF-κB activation, IκBα degradation, IκB kinase activation, and macrophage chemotactic protein-1 expression in the HSF-1-/- cells compared to the HSF-1+/+ cells. These data demonstrate that heat shock inhibits activation of the NF-κB/IκBα pathway and NF-κB-dependent gene expression in the absence of an intact heat shock response.

AB - The heat shock response is known to inhibit NF-κB activation and NF-κB-dependent gene expression. Herein we determined if cells lacking heat shock factor-1 (HSF-1), the major transcription factor regulating heat shock protein gene expression, have an altered ability to modulate NF-κB activation. Embryonic fibroblasts from HSF-1-null mutant mice (HSF-1-/- cells) had a drastically reduced ability to express heat shock protein-70 in response to heat shock, compared to embryonic fibroblasts from wild-type mice (HSF+/+ cells). There was no difference, however, in the ability of heat shock to inhibit TNFα-mediated NF-κB activation, IκBα degradation, IκB kinase activation, and macrophage chemotactic protein-1 expression in the HSF-1-/- cells compared to the HSF-1+/+ cells. These data demonstrate that heat shock inhibits activation of the NF-κB/IκBα pathway and NF-κB-dependent gene expression in the absence of an intact heat shock response.

KW - Cellular activation

KW - Costimulation

KW - Heat shock

KW - Heat shock factor

KW - Heat shock proteins

KW - Iκ kinase

KW - IκBα

KW - NF-κB

KW - Transcription factors

UR - http://www.scopus.com/inward/record.url?scp=0036290257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036290257&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2002.6470

DO - 10.1006/bbrc.2002.6470

M3 - Article

C2 - 11855810

AN - SCOPUS:0036290257

VL - 291

SP - 453

EP - 457

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -