Heat shock proteins are differentially expressed in human gastrointestinal cancers

J. A. Ehrenfried, B. E. Herron, Courtney Townsend, B. M. Evers

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The heat shock proteins (Hsp) are stress-responsive genes present in all species; increases of Hsp can confer chemotherapeutic resistance to certain cancers. The purpose of this study was to determine Hsp expression in human gastric, pancreatic and colon cancers. Gastric (n = 3), pancreatic (n = 6) and colon (n = 8) cancers were extracted for RNA and protein, and Northern and Western blots performed. We found that hsp70 and hsp27 mRNA levels were differentially expressed in the gastrointestinal cancers; mRNA expression closely correlated with protein levels suggesting regulation at the level of transcription. In addition, Hsp90 and Rip proteins were constitutively expressed in the gastrointestinal cancers. We conclude that the Hsp are differentially expressed in human gastric, pancreatic and colon cancers; these increases in Hsp occur constitutively and are not the result of physiological or environmental stresses. Increases of Hsp expression in cancer cells may enhance resistance and account for the altered sensitivity of certain gastrointestinal cancers to chemotherapeutic agents.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalSurgical Oncology
Volume4
Issue number4
StatePublished - 1995

Fingerprint

Gastrointestinal Neoplasms
Heat-Shock Proteins
Pancreatic Neoplasms
Colonic Neoplasms
Stomach Neoplasms
Neoplasms
Messenger RNA
Proteins
Northern Blotting
Stomach
Colon
Western Blotting
RNA
Genes

Keywords

  • Gastrointestinal cancers
  • Heat shock proteins

ASJC Scopus subject areas

  • Oncology
  • Surgery

Cite this

Ehrenfried, J. A., Herron, B. E., Townsend, C., & Evers, B. M. (1995). Heat shock proteins are differentially expressed in human gastrointestinal cancers. Surgical Oncology, 4(4), 197-203.

Heat shock proteins are differentially expressed in human gastrointestinal cancers. / Ehrenfried, J. A.; Herron, B. E.; Townsend, Courtney; Evers, B. M.

In: Surgical Oncology, Vol. 4, No. 4, 1995, p. 197-203.

Research output: Contribution to journalArticle

Ehrenfried, JA, Herron, BE, Townsend, C & Evers, BM 1995, 'Heat shock proteins are differentially expressed in human gastrointestinal cancers', Surgical Oncology, vol. 4, no. 4, pp. 197-203.
Ehrenfried, J. A. ; Herron, B. E. ; Townsend, Courtney ; Evers, B. M. / Heat shock proteins are differentially expressed in human gastrointestinal cancers. In: Surgical Oncology. 1995 ; Vol. 4, No. 4. pp. 197-203.
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