Heat stress modulates hepatocyte membrane proteins during endotoxemia

Rene Przkora, Martin Haslbeck, Marc G. Jeschke, Christoph Heyde, Wolfgang Ertel, David Herndon, Ulrich Bolder

Research output: Contribution to journalArticle

Abstract

Background: Hepatic failure following sepsis is one of the important features of burns. Studies have shown that in septic rats, heat stress (HS) has a protective effect on bile acid transporters in hepatocyte membranes. This study investigates the influence of HS on hepatocyte membrane proteins during endotoxemia using 2D gel electrophoresis. Methods: Endotoxemia in rats was induced by intraperitoneal injection of Escherichia coli lipopolysaccharide (LPS) (n = 24), while control rats (n = 24) received saline. Twelve rats from each group were exposed to HS 2 h prior to LPS or saline injection by external warming to 42 °C for 10 min and 12 rats in each group were exposed to ambient temperature 2 h prior to LPS or saline injection. Membrane fractions were extracted 12, 24 and 72 h after LPS or saline treatment. Extracted proteins were separated using 2D gel electrophoresis. The most dominant spots were analyzed by MALDI-TOF-MS. Results: Two-dimensional gel electrophoresis differentially identified expressed proteins in all treatment groups. The majority of the spots developed 24 h after injection. Membrane proteins; Wnt 13, ribosomal protein L14, VLCAD, BHMT and HIT-40 were found only in HS-LPS. Protein profiles of the groups returned to normal after 72 h. Conclusion: We propose that HS during endotoxemia changes hepatic membrane proteins expression, which are involved in metabolism.

Original languageEnglish
Pages (from-to)628-633
Number of pages6
JournalBurns
Volume33
Issue number5
DOIs
StatePublished - Aug 2007

Fingerprint

Endotoxemia
Lipopolysaccharides
Hepatocytes
Membrane Proteins
Hot Temperature
Electrophoresis, Gel, Two-Dimensional
Injections
Long-Chain Acyl-CoA Dehydrogenase
Proteins
Membranes
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Liver Failure
Intraperitoneal Injections
Burns
Sepsis
Escherichia coli
Temperature
Liver
Therapeutics

Keywords

  • 2D gel electrophoresis
  • Burn
  • Endotoxemia
  • Heat stress
  • Hepatic failure
  • MALDI-TOF-MS
  • Membrane proteins

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Przkora, R., Haslbeck, M., Jeschke, M. G., Heyde, C., Ertel, W., Herndon, D., & Bolder, U. (2007). Heat stress modulates hepatocyte membrane proteins during endotoxemia. Burns, 33(5), 628-633. https://doi.org/10.1016/j.burns.2006.08.016

Heat stress modulates hepatocyte membrane proteins during endotoxemia. / Przkora, Rene; Haslbeck, Martin; Jeschke, Marc G.; Heyde, Christoph; Ertel, Wolfgang; Herndon, David; Bolder, Ulrich.

In: Burns, Vol. 33, No. 5, 08.2007, p. 628-633.

Research output: Contribution to journalArticle

Przkora, R, Haslbeck, M, Jeschke, MG, Heyde, C, Ertel, W, Herndon, D & Bolder, U 2007, 'Heat stress modulates hepatocyte membrane proteins during endotoxemia', Burns, vol. 33, no. 5, pp. 628-633. https://doi.org/10.1016/j.burns.2006.08.016
Przkora R, Haslbeck M, Jeschke MG, Heyde C, Ertel W, Herndon D et al. Heat stress modulates hepatocyte membrane proteins during endotoxemia. Burns. 2007 Aug;33(5):628-633. https://doi.org/10.1016/j.burns.2006.08.016
Przkora, Rene ; Haslbeck, Martin ; Jeschke, Marc G. ; Heyde, Christoph ; Ertel, Wolfgang ; Herndon, David ; Bolder, Ulrich. / Heat stress modulates hepatocyte membrane proteins during endotoxemia. In: Burns. 2007 ; Vol. 33, No. 5. pp. 628-633.
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