Helicobacter pylori-induced IL-8 production by gastric epithelial cells up-regulates CD74 expression

Ellen J. Beswick, Soumita Das, Iryna Pinchuk, Patrick Adegboyega, Giovanni Suarez, Yoshio Yamaoka, Victor Reyes

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

CD74, or the class II MHC-associated invariant chain, is best known for the regulation of Ag presentation. However, recent studies have suggested other important roles for this protein in inflammation and cancer studies. We have shown that CD74 is expressed on the surface of gastric cells, and Helicobacter pylori can use this receptor as a point of attachment to gastric epithelial cells, which lead to IL-8 production. This study investigates the ability of H. pylori to up-regulate one of its receptors in vivo and with a variety of gastric epithelial cell lines during infection with H. pylori. CD74 expression was increased dramatically on gastric biopsies from H. pylori-positive patients and gastric cell lines exposed to the bacteria. Gastric cells exposed to H. pylori-conditioned medium revealed that the host cell response was responsible for the up-regulation of CD74. IL-8 was found to up-regulate CD74 cell surface expression because blocking IL-8Rs or neutralizing IL-8 with Abs counteracted the increased expression of CD74 observed during infection with H. pylori. These studies demonstrate how H. pylori up-regulates one of its own receptors via an autocrine mechanism involving one of the products induced from host cells.

Original languageEnglish (US)
Pages (from-to)171-176
Number of pages6
JournalJournal of Immunology
Volume175
Issue number1
StatePublished - Jul 1 2005

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Interleukin-8
Helicobacter pylori
Stomach
Up-Regulation
Epithelial Cells
Cell Line
Conditioned Culture Medium
Infection
Inflammation
Bacteria
Biopsy
Neoplasms
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Beswick, E. J., Das, S., Pinchuk, I., Adegboyega, P., Suarez, G., Yamaoka, Y., & Reyes, V. (2005). Helicobacter pylori-induced IL-8 production by gastric epithelial cells up-regulates CD74 expression. Journal of Immunology, 175(1), 171-176.

Helicobacter pylori-induced IL-8 production by gastric epithelial cells up-regulates CD74 expression. / Beswick, Ellen J.; Das, Soumita; Pinchuk, Iryna; Adegboyega, Patrick; Suarez, Giovanni; Yamaoka, Yoshio; Reyes, Victor.

In: Journal of Immunology, Vol. 175, No. 1, 01.07.2005, p. 171-176.

Research output: Contribution to journalArticle

Beswick, EJ, Das, S, Pinchuk, I, Adegboyega, P, Suarez, G, Yamaoka, Y & Reyes, V 2005, 'Helicobacter pylori-induced IL-8 production by gastric epithelial cells up-regulates CD74 expression', Journal of Immunology, vol. 175, no. 1, pp. 171-176.
Beswick EJ, Das S, Pinchuk I, Adegboyega P, Suarez G, Yamaoka Y et al. Helicobacter pylori-induced IL-8 production by gastric epithelial cells up-regulates CD74 expression. Journal of Immunology. 2005 Jul 1;175(1):171-176.
Beswick, Ellen J. ; Das, Soumita ; Pinchuk, Iryna ; Adegboyega, Patrick ; Suarez, Giovanni ; Yamaoka, Yoshio ; Reyes, Victor. / Helicobacter pylori-induced IL-8 production by gastric epithelial cells up-regulates CD74 expression. In: Journal of Immunology. 2005 ; Vol. 175, No. 1. pp. 171-176.
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