Helicobacter pylori modulates lymphoepithelial cell interactions leading to epithelial cell damage through Fas/Fas ligand interactions

Jide Wang, Xuejun Fan, Catharlna Lindholm, Michael Bennett, Joe O'Connoll, Fergus Shanahan, Edward G. Brooks, Victor Reyes, Peter B. Ernst

Research output: Contribution to journalArticle

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Abstract

Helicobacter pylori causes a common chronic infection of humans that leads to epithelial cell damage. Studies have shown that apoptosis of the gastric epithelium is increased during infection and this response is associated with an expansion of gastric T-helper type 1 (Th1) cells. We report that gastric T cells contribute to apoptosis of the epithelium by a Fas/Fas ligand (FasL) interaction. Fas receptor expression was detected on freshly isolated gastric epithelial cells by flow cytometry and immunohistochemistry, and this level of expression was increased during infection with H. pylori. The expression of Fas receptor on three gastric epithelial cell lines was increased by H. pylori, either alone or in combination with gamma interferon or tumor necrosis factor alpha. The role of Fas in apoptosis of gastric epithelial cell lines was evidenced by DNA fragmentation after cross-linking of Fas with specific antibodies. FasL expression was detected by immunohistochemistry on mononuclear cells in gastric biopsy specimens of infected but not uninfected subjects. Gastric T- cell lines were also shown to express FasL, as evidenced by reverse transcription-PCR and killing of target cells expressing Fas receptor. Moreover, these T-cell lines were capable of killing cultured gastric epithelial target cells and antibodies that block the interaction between Fas receptor and FasL inhibited this cytotoxic activity. These observations demonstrate that local Th1 cells may contribute to the pathogenesis of gastric disease during H. pylori infection by increasing the expression of Fas on gastric epithelial cells and inducing apoptosis through Fas/FasL interactions.

Original languageEnglish (US)
Pages (from-to)4303-4311
Number of pages9
JournalInfection and Immunity
Volume68
Issue number7
DOIs
StatePublished - Jul 2000

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Fas Ligand Protein
Helicobacter pylori
Cell Communication
Stomach
Epithelial Cells
CD95 Antigens
Apoptosis
Cell Line
Th1 Cells
T-Lymphocytes
Epithelium
Infection
Immunohistochemistry
Stomach Diseases
Antibodies
Helicobacter Infections
DNA Fragmentation
Reverse Transcription
Interferon-gamma
Flow Cytometry

ASJC Scopus subject areas

  • Immunology

Cite this

Helicobacter pylori modulates lymphoepithelial cell interactions leading to epithelial cell damage through Fas/Fas ligand interactions. / Wang, Jide; Fan, Xuejun; Lindholm, Catharlna; Bennett, Michael; O'Connoll, Joe; Shanahan, Fergus; Brooks, Edward G.; Reyes, Victor; Ernst, Peter B.

In: Infection and Immunity, Vol. 68, No. 7, 07.2000, p. 4303-4311.

Research output: Contribution to journalArticle

Wang, Jide ; Fan, Xuejun ; Lindholm, Catharlna ; Bennett, Michael ; O'Connoll, Joe ; Shanahan, Fergus ; Brooks, Edward G. ; Reyes, Victor ; Ernst, Peter B. / Helicobacter pylori modulates lymphoepithelial cell interactions leading to epithelial cell damage through Fas/Fas ligand interactions. In: Infection and Immunity. 2000 ; Vol. 68, No. 7. pp. 4303-4311.
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