Hemodynamic and cardiac contractile function during sepsis caused by cecal ligation and puncture in mice.

Weike Tao, Donald J. Deyo, Daniel L. Traber, William E. Johnston, Edward R. Sherwood

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    Sepsis is among the leading causes of death in the critically ill, yet the pathophysiology of sepsis is incompletely understood. Genetically engineered mice offer a unique opportunity to explore the cellular and molecular pathogenesis of sepsis. However, the hemodynamic responses of mice during sepsis are not completely understood because of the difficulty in performing cardiovascular measurements in mice. We used a 1.4-F pressure and conductance catheter to measure hemodynamics in wild-type C57BL/6J mice during sepsis caused by cecal ligation and puncture. Septic mice exhibited significant hypothermia compared with the sham group. In addition, there was a progressive decrease in mean arterial blood pressure and systemic vascular resistance in septic mice as well as an increase in stroke volume and cardiac output. Sepsis also caused a significant time-dependent impairment of left ventricular function as indicated by decreased dp/dtmax and dp/dtmin. The slope of end systolic pressure volume relationship also decreased over time, as did the time varying maximum elastance and preload-recruitable stroke work of the left ventricle. In conclusion, septic mice exhibit hemodynamic alterations during sepsis that are similar to those observed in humans. The miniaturized conductance catheter allows for effective measurements of hemodynamic function in septic mice and provides measurements that cannot be obtained using other cardiovascular monitoring techniques.

    Original languageEnglish (US)
    Pages (from-to)31-37
    Number of pages7
    JournalShock (Augusta, Ga.)
    Volume21
    Issue number1
    StatePublished - Jan 2004

    Fingerprint

    Punctures
    Ligation
    Sepsis
    Hemodynamics
    Arterial Pressure
    Catheters
    Hypothermia
    Inbred C57BL Mouse
    Left Ventricular Function
    Critical Illness
    Cardiac Output
    Vascular Resistance
    Stroke Volume
    Heart Ventricles
    Cause of Death
    Stroke
    Blood Pressure
    Pressure

    ASJC Scopus subject areas

    • Critical Care and Intensive Care Medicine
    • Physiology

    Cite this

    Tao, W., Deyo, D. J., Traber, D. L., Johnston, W. E., & Sherwood, E. R. (2004). Hemodynamic and cardiac contractile function during sepsis caused by cecal ligation and puncture in mice. Shock (Augusta, Ga.), 21(1), 31-37.

    Hemodynamic and cardiac contractile function during sepsis caused by cecal ligation and puncture in mice. / Tao, Weike; Deyo, Donald J.; Traber, Daniel L.; Johnston, William E.; Sherwood, Edward R.

    In: Shock (Augusta, Ga.), Vol. 21, No. 1, 01.2004, p. 31-37.

    Research output: Contribution to journalArticle

    Tao, W, Deyo, DJ, Traber, DL, Johnston, WE & Sherwood, ER 2004, 'Hemodynamic and cardiac contractile function during sepsis caused by cecal ligation and puncture in mice.', Shock (Augusta, Ga.), vol. 21, no. 1, pp. 31-37.
    Tao, Weike ; Deyo, Donald J. ; Traber, Daniel L. ; Johnston, William E. ; Sherwood, Edward R. / Hemodynamic and cardiac contractile function during sepsis caused by cecal ligation and puncture in mice. In: Shock (Augusta, Ga.). 2004 ; Vol. 21, No. 1. pp. 31-37.
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