Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury

Florian Simon, Riccardo Giudici, Cuong Nguyen Duy, Hubert Schelzig, Sükrü Öter, Michael Gröger, Ulrich Wachter, Josef Vogt, Günter Speit, Csaba Szabó, Peter Radermacher, Enrico Calzia

    Research output: Contribution to journalArticle

    76 Scopus citations

    Abstract

    In awake spontaneously breathing mice, inhaling gaseous hydrogen sulfide (H2S) produced a "suspended animation-like" metabolic status with hypothermia and reduced O2 demand, thus protecting from lethal hypoxia. Murine models may be questioned, however, because due to their large surface area/mass ratio, rodents can rapidly drop their core temperature. Therefore, we investigated whether intravenous H2S (Na2S, sodium sulfide) would induce a comparable metabolic response in anesthetized and mechanically ventilated pigs. Because H2S was reported to improve heart function after myocardial ischemia, we also investigated whether sulfide would influence the noradrenaline responsiveness during reperfusion after aortic occlusion. After 2 h of i.v. sulfide (0.2 mg·kg-1 followed by 2 mg·kg-1·per h; n = 8) or vehicle (n = 8), animals underwent 30 minutes of aortic occlusion with nitroglycerine, esmolol, and adenosine-5′-triphosphate adjusted to maintain MAP at 80% to 120% of baseline. During reperfusion, noradrenaline was titrated to keep MAP greater than or equal to 80% of this level. Sulfide reduced heart rate and cardiac output without affecting stroke volume, markedly decreased the time and dose of noradrenaline required to maintain hemodynamic targets, and caused a drop in core temperature concomitant with lower O2 uptake and CO2 production. Although arterial PCO2 and acid-base status were comparable, arterial PO2 was lower in the sulfide group at the end of the experiment. Sulfide attenuated the reperfusion-related hyperlactatemia, although glycemia was higher at the end of the experiment. The parameters of inflammation and oxidative stress did not differ. Intravenous sulfide allowed reducing energy expenditure in an anesthetized large-animal model and improved the noradrenaline responsiveness during reperfusion after aortic occlusion. Investigations are warranted, hence, whether it may also protect other organs after I/R injury.

    Original languageEnglish (US)
    Pages (from-to)359-364
    Number of pages6
    JournalShock
    Volume30
    Issue number4
    DOIs
    StatePublished - Oct 2008

    Keywords

    • CO production
    • Glucose
    • Hydrogen sulfide
    • Isoprostane
    • Lactate
    • Mitochondrial function
    • Nitrate
    • Nitrite
    • Noradrenaline
    • O consumption
    • Suspended animation
    • TNF-α

    ASJC Scopus subject areas

    • Emergency Medicine
    • Critical Care and Intensive Care Medicine

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  • Cite this

    Simon, F., Giudici, R., Duy, C. N., Schelzig, H., Öter, S., Gröger, M., Wachter, U., Vogt, J., Speit, G., Szabó, C., Radermacher, P., & Calzia, E. (2008). Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury. Shock, 30(4), 359-364. https://doi.org/10.1097/SHK.0b013e3181674185