Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury

Florian Simon, Riccardo Giudici, Cuong Nguyen Duy, Hubert Schelzig, Sükrü Öter, Michael Gröger, Ulrich Wachter, Josef Vogt, Günter Speit, Csaba Szabo, Peter Radermacher, Enrico Calzia

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

In awake spontaneously breathing mice, inhaling gaseous hydrogen sulfide (H2S) produced a "suspended animation-like" metabolic status with hypothermia and reduced O2 demand, thus protecting from lethal hypoxia. Murine models may be questioned, however, because due to their large surface area/mass ratio, rodents can rapidly drop their core temperature. Therefore, we investigated whether intravenous H2S (Na2S, sodium sulfide) would induce a comparable metabolic response in anesthetized and mechanically ventilated pigs. Because H2S was reported to improve heart function after myocardial ischemia, we also investigated whether sulfide would influence the noradrenaline responsiveness during reperfusion after aortic occlusion. After 2 h of i.v. sulfide (0.2 mg·kg-1 followed by 2 mg·kg-1·per h; n = 8) or vehicle (n = 8), animals underwent 30 minutes of aortic occlusion with nitroglycerine, esmolol, and adenosine-5′-triphosphate adjusted to maintain MAP at 80% to 120% of baseline. During reperfusion, noradrenaline was titrated to keep MAP greater than or equal to 80% of this level. Sulfide reduced heart rate and cardiac output without affecting stroke volume, markedly decreased the time and dose of noradrenaline required to maintain hemodynamic targets, and caused a drop in core temperature concomitant with lower O2 uptake and CO2 production. Although arterial PCO2 and acid-base status were comparable, arterial PO2 was lower in the sulfide group at the end of the experiment. Sulfide attenuated the reperfusion-related hyperlactatemia, although glycemia was higher at the end of the experiment. The parameters of inflammation and oxidative stress did not differ. Intravenous sulfide allowed reducing energy expenditure in an anesthetized large-animal model and improved the noradrenaline responsiveness during reperfusion after aortic occlusion. Investigations are warranted, hence, whether it may also protect other organs after I/R injury.

Original languageEnglish (US)
Pages (from-to)359-364
Number of pages6
JournalShock
Volume30
Issue number4
DOIs
StatePublished - Oct 2008
Externally publishedYes

Fingerprint

Hydrogen Sulfide
Sulfides
Reperfusion Injury
Swine
Hemodynamics
Reperfusion
Norepinephrine
Temperature
Nitroglycerin
Hypothermia
Cardiac Output
Stroke Volume
Inhalation
Energy Metabolism
Myocardial Ischemia
Rodentia
Respiration
Oxidative Stress
Animal Models
Adenosine Triphosphate

Keywords

  • CO production
  • Glucose
  • Hydrogen sulfide
  • Isoprostane
  • Lactate
  • Mitochondrial function
  • Nitrate
  • Nitrite
  • Noradrenaline
  • O consumption
  • Suspended animation
  • TNF-α

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Simon, F., Giudici, R., Duy, C. N., Schelzig, H., Öter, S., Gröger, M., ... Calzia, E. (2008). Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury. Shock, 30(4), 359-364. https://doi.org/10.1097/SHK.0b013e3181674185

Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury. / Simon, Florian; Giudici, Riccardo; Duy, Cuong Nguyen; Schelzig, Hubert; Öter, Sükrü; Gröger, Michael; Wachter, Ulrich; Vogt, Josef; Speit, Günter; Szabo, Csaba; Radermacher, Peter; Calzia, Enrico.

In: Shock, Vol. 30, No. 4, 10.2008, p. 359-364.

Research output: Contribution to journalArticle

Simon, F, Giudici, R, Duy, CN, Schelzig, H, Öter, S, Gröger, M, Wachter, U, Vogt, J, Speit, G, Szabo, C, Radermacher, P & Calzia, E 2008, 'Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury', Shock, vol. 30, no. 4, pp. 359-364. https://doi.org/10.1097/SHK.0b013e3181674185
Simon, Florian ; Giudici, Riccardo ; Duy, Cuong Nguyen ; Schelzig, Hubert ; Öter, Sükrü ; Gröger, Michael ; Wachter, Ulrich ; Vogt, Josef ; Speit, Günter ; Szabo, Csaba ; Radermacher, Peter ; Calzia, Enrico. / Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury. In: Shock. 2008 ; Vol. 30, No. 4. pp. 359-364.
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