Hemostatic/fibrinolytic protein changes in C3H/HeN mice infected with Rickettsia conorii

A model for Rocky Mountain spotted fever

A. H. Schmaier, S. Srikanth, M. T. Elghetany, D. Normolle, S. Gokhale, H. M. Feng, David Walker

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Changes in plasma hemostatic and fibrinolytic proteins were determined during courses of a murine model of fatal and non-fatal Rocky Mountain spotted fever. C3H/HeN mice were infected with Rickettsia conorii and coagulation and histopathologic studies were performed at prescribed periods of time. A significant decrease in plasma factor VIII activity and rise in plasma factor V procoagulant activity correlated with a fatal infection. Factor VII levels were unchanged; factor XI levels dropped early in the course in the lethally infected animals, but returned to normal. Factor XII, high molecular weight kininogen, and prekallikrein levels were unchanged by the sublethal infection. Prekallikrein levels fell during the lethal infection. Antithrombin concentrations were decreased significantly in all animals, but plasma plasminogen levels did not change in either group of animals. Non-occlusive thrombi were microscopically observed rarely and only in animals surviving a sublethal infection. A fall in tissue plasminogen activator activity and a rise in plasminogen activator inhibitor activity highly correlated with a lethal outcome. Lethal infection with R. conorii is associated with primary endothelial cell injury resulting in decreased tissue plasminogen activator and increased plasminogen activator inhibitor.

Original languageEnglish (US)
Pages (from-to)871-879
Number of pages9
JournalThrombosis and Haemostasis
Volume86
Issue number3
StatePublished - 2001
Externally publishedYes

Fingerprint

Rickettsia conorii
Rocky Mountain Spotted Fever
Inbred C3H Mouse
Hemostatics
Prekallikrein
Plasminogen Inactivators
Infection
Tissue Plasminogen Activator
Proteins
High Molecular Weight Kininogens
Factor XI
Factor XII
Factor VII
Antithrombins
Plasminogen
Factor VIII
Thrombosis
Endothelial Cells
Wounds and Injuries

Keywords

  • Endothelium
  • Plasminogen activator inhibitor
  • Rickettsial disease
  • Rocky Mountain spotted fever
  • Tissue plasminogen activator

ASJC Scopus subject areas

  • Hematology

Cite this

Schmaier, A. H., Srikanth, S., Elghetany, M. T., Normolle, D., Gokhale, S., Feng, H. M., & Walker, D. (2001). Hemostatic/fibrinolytic protein changes in C3H/HeN mice infected with Rickettsia conorii: A model for Rocky Mountain spotted fever. Thrombosis and Haemostasis, 86(3), 871-879.

Hemostatic/fibrinolytic protein changes in C3H/HeN mice infected with Rickettsia conorii : A model for Rocky Mountain spotted fever. / Schmaier, A. H.; Srikanth, S.; Elghetany, M. T.; Normolle, D.; Gokhale, S.; Feng, H. M.; Walker, David.

In: Thrombosis and Haemostasis, Vol. 86, No. 3, 2001, p. 871-879.

Research output: Contribution to journalArticle

Schmaier, AH, Srikanth, S, Elghetany, MT, Normolle, D, Gokhale, S, Feng, HM & Walker, D 2001, 'Hemostatic/fibrinolytic protein changes in C3H/HeN mice infected with Rickettsia conorii: A model for Rocky Mountain spotted fever', Thrombosis and Haemostasis, vol. 86, no. 3, pp. 871-879.
Schmaier, A. H. ; Srikanth, S. ; Elghetany, M. T. ; Normolle, D. ; Gokhale, S. ; Feng, H. M. ; Walker, David. / Hemostatic/fibrinolytic protein changes in C3H/HeN mice infected with Rickettsia conorii : A model for Rocky Mountain spotted fever. In: Thrombosis and Haemostasis. 2001 ; Vol. 86, No. 3. pp. 871-879.
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