Hepatic protein kinase C is not activated despite high intracellular 1,2-sn-diacylglycerol in obese Zucker rats

Ok Ho Shin, Kerry Ann Da Costa, Mei Heng Mar, Steven H. Zeisel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

High intracellular 1,2,-sn-diacylglycerol (DAG) usually activates protein kinase C (PKC). In choline-deficient Fischer 344 rats, we previously showed that fatty liver was associated with elevated hepatic DAG and sustained activation of PKC. Steatosis is a sequelae of many liver toxins, and we wanted to determine whether fatty liver is always associated with accumulation of DAG with activation of PKC. Obese Zucker rats had 11-fold more triacylglycerol in their livers and 2-fold more DAG in their hepatic plasma membrane than did lean control Zucker rats. However, this increased diacylglycerol was not associated with translocation or activation of PKC in hepatic plasma membrane (activity in obese rats was 897 pmol/mg protein x min-1 vs. 780 pmol/mg protein x min-1 in lean rats). No differences in PKC isoform expression were detected between obese and lean rats. In additional studies, we found that choline deficiency in the Zucker rat did not result in activation of PKC in liver, unlike our earlier observations in the choline deficient Fischer rat. This dissociation between fatty liver, DAG accumulation and PKC activation in Zucker rats supports previous reports of abnormalities in PKC signaling in this strain of rats.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1358
Issue number1
DOIs
StatePublished - Aug 21 1997
Externally publishedYes

Fingerprint

Zucker Rats
Protein Kinase C
Liver
Diglycerides
Fatty Liver
Inbred F344 Rats
Choline
Choline Deficiency
Cell Membrane
Diacylglycerol Kinase
1,2-diacylglycerol
Protein Isoforms
Triglycerides
Proteins

Keywords

  • Diacylglycerol
  • Liver
  • Protein kinase C
  • Zucker rat

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Hepatic protein kinase C is not activated despite high intracellular 1,2-sn-diacylglycerol in obese Zucker rats. / Shin, Ok Ho; Da Costa, Kerry Ann; Mar, Mei Heng; Zeisel, Steven H.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1358, No. 1, 21.08.1997, p. 72-78.

Research output: Contribution to journalArticle

Shin, Ok Ho ; Da Costa, Kerry Ann ; Mar, Mei Heng ; Zeisel, Steven H. / Hepatic protein kinase C is not activated despite high intracellular 1,2-sn-diacylglycerol in obese Zucker rats. In: Biochimica et Biophysica Acta - Molecular Cell Research. 1997 ; Vol. 1358, No. 1. pp. 72-78.
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