Hepatitis C virus core protein increases mitochondrial ROS production by stimulation of Ca2+ uniporter activity

Yanchun Li, Darren F. Boehning, Ting Qian, Vsevolod L. Popov, Steven A. Weinman

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

Many viruses have evolved mechanisms to alter mitochondrial function. The hepatitis C virus (HCV) produces a viral core protein that targets to mitochondria and increases Ca2+-dependent ROS production. The aim of this study was to determine whether core's effects are mediated by changes in mitochondrial Ca2+ uptake. Core expression caused enhanced mitochondrial Ca2+ uptake in response to ER Ca2+ release induced by thapsigargin or ATP. It also increased mitochondrial superoxide production and mitochondrial permeability transition (MPT). Incubating mouse liver mitochondria with an HCV core (100 ng/mg) in vitro increased Ca 2+ entry rate by ∼ 2-fold. Entry was entirely inhibited by the mitochondrial Ca2+ uniporter inhibitor, Ru-360, but not influenced by an Na+/Ca2+ exchanger inhibitor or ROS scavengers. These results indicate that core directly increases mitochondrial Ca2+ uptake via a primary effect on the uniporter. This enhanced the ability of mitochondria to sequester Ca2+ in response to ER Ca2+ release, and increased mitochondrial ROS production and MPT. Thus, the mitochondrial Ca2+ uniporter is a newly identified target for viral modification of cell function.

Original languageEnglish (US)
Pages (from-to)2474-2485
Number of pages12
JournalFASEB Journal
Volume21
Issue number10
DOIs
StatePublished - Aug 1 2007

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Keywords

  • Ca
  • HCV core
  • MAVS
  • Mitochondria cytosolic

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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