Hepatitis C virus envelope proteins bind lactoferrin

Min Kyung Yi, Shuichi Kaneko, Dae Yeul Yu, Seishi Murakami

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) has two envelope proteins, E1 and E2, which form a heterooligomer. During dissection of interacting regions of HCV E1 and E2, we found the presence of an interfering compound or compounds in skim milk. Here we report that human as well as bovine lactoferrin, a multifunctional immunomodulator, binds two HCV envelope proteins. As determined by far- Western blotting, the bacterially expressed E1 and E2 could bind lactoferrin in human milk directly separated or immunopurified and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The bindings of lactoferrin and HCV envelope proteins in vitro were confirmed by another method, the pull-down assay, with immunoprecipitated lactoferrin-bound protein A resin. By the same assay, mammal-expressed recombinant E1 and E2 and were also demonstrated to bind human lactoferrin efficiently in vitro. Direct interaction between E2 and lactoferrin was proved in vivo, since anti- human lactoferrin antibody efficiently coimmunoprecipitated with secreted and intracellular forms of the E2 protein, but no glutathione S-transferase (GST), from lysates of HepG2 cells transiently cotransfected with the expression plasmids of human lactoferrin and gE2t-GST (the N-terminal two- thirds of E2 fused to GST) or GST. The N-terminal loop of lactoferrin, the region important for the antibacterial activity, has only a little role in the binding ability to HCV E2 but affected the secretion or stability of lactoferrin. Taken together, these results indicate the specific interaction between lactoferrin and HCV envelope proteins in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)5997-6002
Number of pages6
JournalJournal of Virology
Volume71
Issue number8
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

Viral Envelope Proteins
Lactoferrin
Hepatitis C virus
lactoferrin
Hepacivirus
proteins
Glutathione Transferase
glutathione transferase
Far-Western Blotting
immunomodulators
Staphylococcal Protein A
Hep G2 Cells
Immunologic Factors
binding capacity
assays
Human Milk
skim milk
breast milk
Sodium Dodecyl Sulfate
resins

ASJC Scopus subject areas

  • Immunology

Cite this

Yi, M. K., Kaneko, S., Yu, D. Y., & Murakami, S. (1997). Hepatitis C virus envelope proteins bind lactoferrin. Journal of Virology, 71(8), 5997-6002.

Hepatitis C virus envelope proteins bind lactoferrin. / Yi, Min Kyung; Kaneko, Shuichi; Yu, Dae Yeul; Murakami, Seishi.

In: Journal of Virology, Vol. 71, No. 8, 08.1997, p. 5997-6002.

Research output: Contribution to journalArticle

Yi, MK, Kaneko, S, Yu, DY & Murakami, S 1997, 'Hepatitis C virus envelope proteins bind lactoferrin', Journal of Virology, vol. 71, no. 8, pp. 5997-6002.
Yi MK, Kaneko S, Yu DY, Murakami S. Hepatitis C virus envelope proteins bind lactoferrin. Journal of Virology. 1997 Aug;71(8):5997-6002.
Yi, Min Kyung ; Kaneko, Shuichi ; Yu, Dae Yeul ; Murakami, Seishi. / Hepatitis C virus envelope proteins bind lactoferrin. In: Journal of Virology. 1997 ; Vol. 71, No. 8. pp. 5997-6002.
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