Heritable susceptibility for colitis in mice induced by IL-10 deficiency

Ian J. Bristol, Mark A. Farmer, Yingzi Cong, Xin Xiao Zheng, Terry B. Strom, Charles O. Elson, John P. Sundberg, Edward H. Leiter

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Severity of inflammatory bowel disease in IL-10 gene-targeted mice is in part determined by genetic back-ground. In the current study, a targeted IL-10 gene was transferred into the C3H/HeJBir substrain, known to exhibit high T-cell and B-cell responses to enteric flora, and to be highly sensitive to colitigenic stress. IL-10-deficient C3H/HeJBir mice developed early onset colitis in contrast to IL-10-deficient C57BL/6J congenic mice. Histopathologic analysis of disease in C3H/HeJBir.Il10-/- and C57BL/6J.Il10-/- mice showed significant differences at all ages studied. Hybrids of these congenic strains (F1.Il10-/-) were produced to study the mode of inheritance as well as subphenotypes that correlated with his-topathology. Lesions in F1 mice were intermediate between parental strains. C3H-contributed subphenotypes that correlated best with histopathology were peripheral blood granulocyte percentage, serum amyloid A concentration, spleen weight/body weight ratio, and mesenteric lymph node weight/ body weight ratio. Neither enhanced humoral immunity (secretory IgA; anti-Escherichia coli cellular membrane Ig) characteristic of C3H/HeJBir, nor T-cell percentages in peripheral blood correlated as well. This study represents a necessary step in elucidating murine genetic modifiers controlling colitis sensitivity.

Original languageEnglish (US)
Pages (from-to)290-302
Number of pages13
JournalInflammatory Bowel Diseases
Volume6
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

Colitis
Interleukin-10
Body Weight
Congenic Mice
T-Lymphocytes
Serum Amyloid A Protein
Weights and Measures
Secretory Immunoglobulin A
Inbred C3H Mouse
Humoral Immunity
Inflammatory Bowel Diseases
Granulocytes
Genes
B-Lymphocytes
Spleen
Lymph Nodes
Escherichia coli
Membranes

Keywords

  • Colitis
  • Genetics
  • Interleukin 10
  • Mice
  • Pathology

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Bristol, I. J., Farmer, M. A., Cong, Y., Zheng, X. X., Strom, T. B., Elson, C. O., ... Leiter, E. H. (2000). Heritable susceptibility for colitis in mice induced by IL-10 deficiency. Inflammatory Bowel Diseases, 6(4), 290-302.

Heritable susceptibility for colitis in mice induced by IL-10 deficiency. / Bristol, Ian J.; Farmer, Mark A.; Cong, Yingzi; Zheng, Xin Xiao; Strom, Terry B.; Elson, Charles O.; Sundberg, John P.; Leiter, Edward H.

In: Inflammatory Bowel Diseases, Vol. 6, No. 4, 2000, p. 290-302.

Research output: Contribution to journalArticle

Bristol, IJ, Farmer, MA, Cong, Y, Zheng, XX, Strom, TB, Elson, CO, Sundberg, JP & Leiter, EH 2000, 'Heritable susceptibility for colitis in mice induced by IL-10 deficiency', Inflammatory Bowel Diseases, vol. 6, no. 4, pp. 290-302.
Bristol IJ, Farmer MA, Cong Y, Zheng XX, Strom TB, Elson CO et al. Heritable susceptibility for colitis in mice induced by IL-10 deficiency. Inflammatory Bowel Diseases. 2000;6(4):290-302.
Bristol, Ian J. ; Farmer, Mark A. ; Cong, Yingzi ; Zheng, Xin Xiao ; Strom, Terry B. ; Elson, Charles O. ; Sundberg, John P. ; Leiter, Edward H. / Heritable susceptibility for colitis in mice induced by IL-10 deficiency. In: Inflammatory Bowel Diseases. 2000 ; Vol. 6, No. 4. pp. 290-302.
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