Herpes simplex virus latency in SV40 hamster tumour cells

Istvan Boldogh, L. Geder, L. Vaczi

Research output: Contribution to journalArticle

Abstract

Incubation of SV40 tumor cell cultures with herpes simplex virus type 2 (HSV 2) at 40°C resulted in rapidly growing cells which survived the infection and lacked both virus cytopathology and synthesis of detectable infectious virus. Following transfer to 37°C, infectious HSV 2 reappeared and persistent infection of cells developed without complete destruction of the cell line. There was, however, a delay of at least 8 to 22 days following transfer to 37°C before infectious virus could be detected. HSV specific immunofluorescent antigens were present in every cell during latency. The reappearance of infectious HSV 2 went parallel with focal cytopathogenic changes. The period between the disappearance and reappearance of infectious virus is considered the latent period. Cultures in this period were less sensitive to superinfection with herpes simplex virus type 2 than control cultures. The reappearance of infectious HSV 2 after prolonged incubation of infected culture at 40°C and 37°C indicates that HSV 2 can remain associated with the cells in a non infectious form for long periods without being degraded.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalActa Microbiologica Academiae Scientiarum Hungaricae
Volume20
Issue number2
StatePublished - 1973
Externally publishedYes

Fingerprint

Virus Latency
Human Herpesvirus 2
Simplexvirus
Cricetinae
Viruses
Neoplasms
Superinfection
Infection
Cell Culture Techniques
Antigens
Cell Line

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Herpes simplex virus latency in SV40 hamster tumour cells. / Boldogh, Istvan; Geder, L.; Vaczi, L.

In: Acta Microbiologica Academiae Scientiarum Hungaricae, Vol. 20, No. 2, 1973, p. 127-134.

Research output: Contribution to journalArticle

@article{1fdc304fc74a4de6a8d49ce29299b7dc,
title = "Herpes simplex virus latency in SV40 hamster tumour cells",
abstract = "Incubation of SV40 tumor cell cultures with herpes simplex virus type 2 (HSV 2) at 40°C resulted in rapidly growing cells which survived the infection and lacked both virus cytopathology and synthesis of detectable infectious virus. Following transfer to 37°C, infectious HSV 2 reappeared and persistent infection of cells developed without complete destruction of the cell line. There was, however, a delay of at least 8 to 22 days following transfer to 37°C before infectious virus could be detected. HSV specific immunofluorescent antigens were present in every cell during latency. The reappearance of infectious HSV 2 went parallel with focal cytopathogenic changes. The period between the disappearance and reappearance of infectious virus is considered the latent period. Cultures in this period were less sensitive to superinfection with herpes simplex virus type 2 than control cultures. The reappearance of infectious HSV 2 after prolonged incubation of infected culture at 40°C and 37°C indicates that HSV 2 can remain associated with the cells in a non infectious form for long periods without being degraded.",
author = "Istvan Boldogh and L. Geder and L. Vaczi",
year = "1973",
language = "English (US)",
volume = "20",
pages = "127--134",
journal = "Acta Microbiologica et Immunologica Hungarica",
issn = "1217-8950",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Herpes simplex virus latency in SV40 hamster tumour cells

AU - Boldogh, Istvan

AU - Geder, L.

AU - Vaczi, L.

PY - 1973

Y1 - 1973

N2 - Incubation of SV40 tumor cell cultures with herpes simplex virus type 2 (HSV 2) at 40°C resulted in rapidly growing cells which survived the infection and lacked both virus cytopathology and synthesis of detectable infectious virus. Following transfer to 37°C, infectious HSV 2 reappeared and persistent infection of cells developed without complete destruction of the cell line. There was, however, a delay of at least 8 to 22 days following transfer to 37°C before infectious virus could be detected. HSV specific immunofluorescent antigens were present in every cell during latency. The reappearance of infectious HSV 2 went parallel with focal cytopathogenic changes. The period between the disappearance and reappearance of infectious virus is considered the latent period. Cultures in this period were less sensitive to superinfection with herpes simplex virus type 2 than control cultures. The reappearance of infectious HSV 2 after prolonged incubation of infected culture at 40°C and 37°C indicates that HSV 2 can remain associated with the cells in a non infectious form for long periods without being degraded.

AB - Incubation of SV40 tumor cell cultures with herpes simplex virus type 2 (HSV 2) at 40°C resulted in rapidly growing cells which survived the infection and lacked both virus cytopathology and synthesis of detectable infectious virus. Following transfer to 37°C, infectious HSV 2 reappeared and persistent infection of cells developed without complete destruction of the cell line. There was, however, a delay of at least 8 to 22 days following transfer to 37°C before infectious virus could be detected. HSV specific immunofluorescent antigens were present in every cell during latency. The reappearance of infectious HSV 2 went parallel with focal cytopathogenic changes. The period between the disappearance and reappearance of infectious virus is considered the latent period. Cultures in this period were less sensitive to superinfection with herpes simplex virus type 2 than control cultures. The reappearance of infectious HSV 2 after prolonged incubation of infected culture at 40°C and 37°C indicates that HSV 2 can remain associated with the cells in a non infectious form for long periods without being degraded.

UR - http://www.scopus.com/inward/record.url?scp=0015818112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015818112&partnerID=8YFLogxK

M3 - Article

C2 - 4359669

AN - SCOPUS:0015818112

VL - 20

SP - 127

EP - 134

JO - Acta Microbiologica et Immunologica Hungarica

JF - Acta Microbiologica et Immunologica Hungarica

SN - 1217-8950

IS - 2

ER -