Abstract
The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148% to 170% increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 155-161 |
| Number of pages | 7 |
| Journal | Investigational New Drugs |
| Volume | 7 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Jul 1989 |
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Keywords
- cell killing
- heterogeneity
- kinetics
- polyamines
ASJC Scopus subject areas
- Pharmacology
- Molecular Medicine
Cite this
Heterogeneous survival and cell kinetics responses of human astrocytoma clones to α-difluoromethylornithine in vitro. / Barranco, S. C.; Ford, P. J.; Townsend, Courtney.
In: Investigational New Drugs, Vol. 7, No. 2-3, 07.1989, p. 155-161.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heterogeneous survival and cell kinetics responses of human astrocytoma clones to α-difluoromethylornithine in vitro
AU - Barranco, S. C.
AU - Ford, P. J.
AU - Townsend, Courtney
PY - 1989/7
Y1 - 1989/7
N2 - The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148% to 170% increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.
AB - The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148% to 170% increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.
KW - cell killing
KW - heterogeneity
KW - kinetics
KW - polyamines
UR - http://www.scopus.com/inward/record.url?scp=0024413671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024413671&partnerID=8YFLogxK
U2 - 10.1007/BF00170852
DO - 10.1007/BF00170852
M3 - Article
C2 - 2507473
AN - SCOPUS:0024413671
VL - 7
SP - 155
EP - 161
JO - Investigational New Drugs
JF - Investigational New Drugs
SN - 0167-6997
IS - 2-3
ER -