Heterogeneous survival and cell kinetics responses of human astrocytoma clones to α-difluoromethylornithine in vitro

S. C. Barranco, P. J. Ford, Courtney Townsend

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148% to 170% increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalInvestigational New Drugs
Volume7
Issue number2-3
DOIs
StatePublished - Jul 1989

Fingerprint

Eflornithine
Astrocytoma
Cell Survival
Clone Cells
Polyamines
Putrescine
Spermidine
Survival
In Vitro Techniques
Population

Keywords

  • cell killing
  • heterogeneity
  • kinetics
  • polyamines

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Heterogeneous survival and cell kinetics responses of human astrocytoma clones to α-difluoromethylornithine in vitro. / Barranco, S. C.; Ford, P. J.; Townsend, Courtney.

In: Investigational New Drugs, Vol. 7, No. 2-3, 07.1989, p. 155-161.

Research output: Contribution to journalArticle

@article{cabb88cbb537429b8b4c6733125141ca,
title = "Heterogeneous survival and cell kinetics responses of human astrocytoma clones to α-difluoromethylornithine in vitro",
abstract = "The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148{\%} to 170{\%} increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.",
keywords = "cell killing, heterogeneity, kinetics, polyamines",
author = "Barranco, {S. C.} and Ford, {P. J.} and Courtney Townsend",
year = "1989",
month = "7",
doi = "10.1007/BF00170852",
language = "English (US)",
volume = "7",
pages = "155--161",
journal = "Investigational New Drugs",
issn = "0167-6997",
publisher = "Kluwer Academic Publishers",
number = "2-3",

}

TY - JOUR

T1 - Heterogeneous survival and cell kinetics responses of human astrocytoma clones to α-difluoromethylornithine in vitro

AU - Barranco, S. C.

AU - Ford, P. J.

AU - Townsend, Courtney

PY - 1989/7

Y1 - 1989/7

N2 - The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148% to 170% increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.

AB - The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148% to 170% increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.

KW - cell killing

KW - heterogeneity

KW - kinetics

KW - polyamines

UR - http://www.scopus.com/inward/record.url?scp=0024413671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024413671&partnerID=8YFLogxK

U2 - 10.1007/BF00170852

DO - 10.1007/BF00170852

M3 - Article

C2 - 2507473

AN - SCOPUS:0024413671

VL - 7

SP - 155

EP - 161

JO - Investigational New Drugs

JF - Investigational New Drugs

SN - 0167-6997

IS - 2-3

ER -