Heterogeneous survival and cell kinetics responses of human astrocytoma clones to α-difluoromethylornithine in vitro

S. C. Barranco, P. J. Ford, C. M. Townsend

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The effects of α-difluoromethylornithine (DFMO) on survival, cell kinetics and polyamine levels were studied on two clones of human astrocytoma in vitro. The survival responses were dose and time dependent; and treatments with DFMO which lasted for 72 h resulted in heterogeneous responses with one clone being up to 6 times more sensitive than the other. Shorter treatments produced more uniform killing in the clones. A continuous exposure of the cells to 5 mM DFMO resulted in a rapid decrease in putrescine values in both clones, followed by decreases in the spermidine levels. These effects were closely followed by 148% to 170% increases in cell population doubling times, and a lowering of saturation densities. No clear correlations could be established among baseline polyamine levels and cell kinetics or survival responses to DFMO treatments.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalInvestigational New Drugs
Volume7
Issue number2-3
DOIs
StatePublished - Jul 1989

Keywords

  • cell killing
  • heterogeneity
  • kinetics
  • polyamines

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Pharmacology (medical)

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