Hexameric ring structure of human MCM10 DNA replication factor

Andrei L. Okorokov, Alastair Waugh, Julie Hodgkinson, Andal Murthy, Hye Kyung Hong, Elisabetta Leo, Michael Sherman, Kai Stoeber, Elena V. Orlova, Gareth H. Williams

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The DNA replication factor minichromosome maintenance 10 (MCM10) is a conserved, abundant nuclear protein crucial for origin firing. During the transition from pre-replicative complexes to pre-initiation complexes, MCM10 recruitment to replication origins is required to provide a physical link between the MCM2-7 complex DNA helicase and DNA polymerases. Here, we report the molecular structure of human MCM10 as determined by electron microscopy and single-particle analysis. The MCM10 molecule is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers. This structure, together with biochemical data, suggests that this important protein uses its architecture to provide a docking module for assembly of the molecular machinery required for eukaryotic DNA replication.

Original languageEnglish
Pages (from-to)925-930
Number of pages6
JournalEMBO Reports
Volume8
Issue number10
DOIs
StatePublished - Oct 2007

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DNA Replication
Maintenance
DNA
Minichromosome Maintenance Proteins
DNA Helicases
Replication Origin
DNA-Directed DNA Polymerase
Nuclear Proteins
Molecular Structure
Molecular structure
Electron microscopy
Machinery
Electron Microscopy
Molecules
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Okorokov, A. L., Waugh, A., Hodgkinson, J., Murthy, A., Hong, H. K., Leo, E., ... Williams, G. H. (2007). Hexameric ring structure of human MCM10 DNA replication factor. EMBO Reports, 8(10), 925-930. https://doi.org/10.1038/sj.embor.7401064

Hexameric ring structure of human MCM10 DNA replication factor. / Okorokov, Andrei L.; Waugh, Alastair; Hodgkinson, Julie; Murthy, Andal; Hong, Hye Kyung; Leo, Elisabetta; Sherman, Michael; Stoeber, Kai; Orlova, Elena V.; Williams, Gareth H.

In: EMBO Reports, Vol. 8, No. 10, 10.2007, p. 925-930.

Research output: Contribution to journalArticle

Okorokov, AL, Waugh, A, Hodgkinson, J, Murthy, A, Hong, HK, Leo, E, Sherman, M, Stoeber, K, Orlova, EV & Williams, GH 2007, 'Hexameric ring structure of human MCM10 DNA replication factor', EMBO Reports, vol. 8, no. 10, pp. 925-930. https://doi.org/10.1038/sj.embor.7401064
Okorokov AL, Waugh A, Hodgkinson J, Murthy A, Hong HK, Leo E et al. Hexameric ring structure of human MCM10 DNA replication factor. EMBO Reports. 2007 Oct;8(10):925-930. https://doi.org/10.1038/sj.embor.7401064
Okorokov, Andrei L. ; Waugh, Alastair ; Hodgkinson, Julie ; Murthy, Andal ; Hong, Hye Kyung ; Leo, Elisabetta ; Sherman, Michael ; Stoeber, Kai ; Orlova, Elena V. ; Williams, Gareth H. / Hexameric ring structure of human MCM10 DNA replication factor. In: EMBO Reports. 2007 ; Vol. 8, No. 10. pp. 925-930.
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