High-affinity binding sites for prostaglandin E on human lymphocytes

James Goodwin, Alan Wiik, Mary Lewis, Arthur D. Bankhurst, Ralph C. Williams

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Binding sites on human lymphocytes for prostaglandins were examined by incubating cells with [3H]prostaglandin (PG) A1, E1, E2, F, and F. Specific reversible binding for [3H]PGE1 and E2 was found with a Kd of ~2 × 10-9M and a B max of ~200 binding sites per cell, assuming uniform distribution. We detected no specific binding of [3H]PGA1, F, or F to lymphocytes. Also, the addition of 10- to 1000-fold greater amounts of unlabeled PGA, F, or F did not inhibit the binding of [3H]PGE. The time course of [3H]PGE binding appeared to be bimodal with one component complete within 5 min at 37 °C and another component of binding increasing over a 40-min incubation. We feel that the rapid component of binding may represent cell surface receptors for PGE while the slower component may represent a specific uptake mechanism for PGE into the cell. Glass adherent cells had fewer binding sites than nonadherent cells. Preincubation of the cells overnight resulted in a loss of binding sites.

Original languageEnglish (US)
Pages (from-to)150-159
Number of pages10
JournalCellular Immunology
Volume43
Issue number1
DOIs
StatePublished - Mar 1 1979
Externally publishedYes

Fingerprint

Prostaglandins E
Binding Sites
Lymphocytes
Alprostadil
Prostaglandins A
Cell Surface Receptors
Prostaglandins
Glass

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

High-affinity binding sites for prostaglandin E on human lymphocytes. / Goodwin, James; Wiik, Alan; Lewis, Mary; Bankhurst, Arthur D.; Williams, Ralph C.

In: Cellular Immunology, Vol. 43, No. 1, 01.03.1979, p. 150-159.

Research output: Contribution to journalArticle

Goodwin, James ; Wiik, Alan ; Lewis, Mary ; Bankhurst, Arthur D. ; Williams, Ralph C. / High-affinity binding sites for prostaglandin E on human lymphocytes. In: Cellular Immunology. 1979 ; Vol. 43, No. 1. pp. 150-159.
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