High-affinity binding sites for prostaglandin E on human lymphocytes

James S. Goodwin, Alan Wiik, Mary Lewis, Arthur D. Bankhurst, Ralph C. Williams

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Binding sites on human lymphocytes for prostaglandins were examined by incubating cells with [3H]prostaglandin (PG) A1, E1, E2, F, and F. Specific reversible binding for [3H]PGE1 and E2 was found with a Kd of ~2 × 10-9M and a B max of ~200 binding sites per cell, assuming uniform distribution. We detected no specific binding of [3H]PGA1, F, or F to lymphocytes. Also, the addition of 10- to 1000-fold greater amounts of unlabeled PGA, F, or F did not inhibit the binding of [3H]PGE. The time course of [3H]PGE binding appeared to be bimodal with one component complete within 5 min at 37 °C and another component of binding increasing over a 40-min incubation. We feel that the rapid component of binding may represent cell surface receptors for PGE while the slower component may represent a specific uptake mechanism for PGE into the cell. Glass adherent cells had fewer binding sites than nonadherent cells. Preincubation of the cells overnight resulted in a loss of binding sites.

Original languageEnglish (US)
Pages (from-to)150-159
Number of pages10
JournalCellular Immunology
Volume43
Issue number1
DOIs
StatePublished - Mar 1 1979

ASJC Scopus subject areas

  • Immunology

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