High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles

Michiel Van De Waterbeemd, Kyle L. Fort, Dmitriy Boll, Maria Reinhardt-Szyba, Andrew Routh, Alexander Makarov, Albert J R Heck

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8-to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.

Original languageEnglish (US)
Pages (from-to)283-286
Number of pages4
JournalNature Methods
Volume14
Issue number3
DOIs
StatePublished - Feb 28 2017

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Ribosomes
Mass spectrometers
Viruses
Nucleic Acids
Molecular Biology
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Van De Waterbeemd, M., Fort, K. L., Boll, D., Reinhardt-Szyba, M., Routh, A., Makarov, A., & Heck, A. J. R. (2017). High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles. Nature Methods, 14(3), 283-286. https://doi.org/10.1038/nmeth.4147

High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles. / Van De Waterbeemd, Michiel; Fort, Kyle L.; Boll, Dmitriy; Reinhardt-Szyba, Maria; Routh, Andrew; Makarov, Alexander; Heck, Albert J R.

In: Nature Methods, Vol. 14, No. 3, 28.02.2017, p. 283-286.

Research output: Contribution to journalArticle

Van De Waterbeemd, M, Fort, KL, Boll, D, Reinhardt-Szyba, M, Routh, A, Makarov, A & Heck, AJR 2017, 'High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles', Nature Methods, vol. 14, no. 3, pp. 283-286. https://doi.org/10.1038/nmeth.4147
Van De Waterbeemd M, Fort KL, Boll D, Reinhardt-Szyba M, Routh A, Makarov A et al. High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles. Nature Methods. 2017 Feb 28;14(3):283-286. https://doi.org/10.1038/nmeth.4147
Van De Waterbeemd, Michiel ; Fort, Kyle L. ; Boll, Dmitriy ; Reinhardt-Szyba, Maria ; Routh, Andrew ; Makarov, Alexander ; Heck, Albert J R. / High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles. In: Nature Methods. 2017 ; Vol. 14, No. 3. pp. 283-286.
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