High-frequency leader sequence switching during coronavirus defective interfering RNA replication

Shinji Makino, M. M C Lai

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A system was developed that exploited defective interfering (DI) RNAs of coronavirus to study the role of free leader RNA in RNA replication. A cDNA copy of mouse hepatitis virus DI RNA was placed downstream of the T7 RNA polymerase promoter to generate DI RNAs capable of extremely efficient replication in the presence of a helper virus. We demonstrated that, in the DI RNA-transfected cells, the leader sequence of these DI RNAs was switched to that of the helper virus during one round of replication. This high-frequency leader sequence exchange was not observed if a nine-nucleotide stretch of sequence (UUUAUAAAC) at the junction between the leader and the remaining DI sequence was deleted. This observation suggests that a free leader RNA generated from the genomic RNA of mouse hepatitis virus may participate in the replication of DI RNA.

Original languageEnglish (US)
Pages (from-to)5285-5292
Number of pages8
JournalJournal of Virology
Volume63
Issue number12
StatePublished - 1989
Externally publishedYes

Fingerprint

Coronavirus
Coronavirinae
RNA
Murine hepatitis virus
Helper Viruses
RNA replication
viruses
DNA-directed RNA polymerase
promoter regions
Complementary DNA
genomics
nucleotide sequences

ASJC Scopus subject areas

  • Immunology

Cite this

High-frequency leader sequence switching during coronavirus defective interfering RNA replication. / Makino, Shinji; Lai, M. M C.

In: Journal of Virology, Vol. 63, No. 12, 1989, p. 5285-5292.

Research output: Contribution to journalArticle

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