High-frequency RNA recombination of murine coronaviruses

Shinji Makino, J. G. Keck, S. A. Stohlman, M. M C Lai

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

The RNA genome of coronaviruses consists of a single species of nonsegmented RNA. In this communication, we demonstrate that the RNA genomes of different strains of murine coronaviruses recombine during mixed infection at a very high frequency. Susceptible cells were coinfected with a temperature-sensitive mutant of one strain of mouse hepatitis virus (MHV) and a wild-type virus of a different strain. Of 21 randomly isolated viruses released from the coinfected cells at the nonpermissive temperature, 2 were recombinants which differed in the site of recombination. After three serial passages of the original virus pool derived from the mixed infection, the majority of the progeny viruses were recombinants. These recombinant viruses represented at least five different recombination sites between the two parental MHV strains. Such a high-frequency recombination between nonsegmented RNA genomes of MHV suggests that segmented RNA intermediates might be generated during MHV replication. We propose that the RNA replication of MHV proceeds in a discontinuous and nonprocessive manner, thus generating free segmented RNA intermediates, which could be used in RNA recombination via a copy-choice mechanism.

Original languageEnglish (US)
Pages (from-to)729-737
Number of pages9
JournalJournal of Virology
Volume57
Issue number3
StatePublished - 1986
Externally publishedYes

Fingerprint

Murine coronavirus
Murine hepatitis virus
Coronavirus
Genetic Recombination
RNA
viruses
Viruses
mixed infection
Genome
genome
Coinfection
Mutant Strains Mice
Coronavirinae
Serial Passage
Temperature
virus replication
Virus Replication
temperature
cells

ASJC Scopus subject areas

  • Immunology

Cite this

Makino, S., Keck, J. G., Stohlman, S. A., & Lai, M. M. C. (1986). High-frequency RNA recombination of murine coronaviruses. Journal of Virology, 57(3), 729-737.

High-frequency RNA recombination of murine coronaviruses. / Makino, Shinji; Keck, J. G.; Stohlman, S. A.; Lai, M. M C.

In: Journal of Virology, Vol. 57, No. 3, 1986, p. 729-737.

Research output: Contribution to journalArticle

Makino, S, Keck, JG, Stohlman, SA & Lai, MMC 1986, 'High-frequency RNA recombination of murine coronaviruses', Journal of Virology, vol. 57, no. 3, pp. 729-737.
Makino S, Keck JG, Stohlman SA, Lai MMC. High-frequency RNA recombination of murine coronaviruses. Journal of Virology. 1986;57(3):729-737.
Makino, Shinji ; Keck, J. G. ; Stohlman, S. A. ; Lai, M. M C. / High-frequency RNA recombination of murine coronaviruses. In: Journal of Virology. 1986 ; Vol. 57, No. 3. pp. 729-737.
@article{376174977de04e93bb572e56119735ee,
title = "High-frequency RNA recombination of murine coronaviruses",
abstract = "The RNA genome of coronaviruses consists of a single species of nonsegmented RNA. In this communication, we demonstrate that the RNA genomes of different strains of murine coronaviruses recombine during mixed infection at a very high frequency. Susceptible cells were coinfected with a temperature-sensitive mutant of one strain of mouse hepatitis virus (MHV) and a wild-type virus of a different strain. Of 21 randomly isolated viruses released from the coinfected cells at the nonpermissive temperature, 2 were recombinants which differed in the site of recombination. After three serial passages of the original virus pool derived from the mixed infection, the majority of the progeny viruses were recombinants. These recombinant viruses represented at least five different recombination sites between the two parental MHV strains. Such a high-frequency recombination between nonsegmented RNA genomes of MHV suggests that segmented RNA intermediates might be generated during MHV replication. We propose that the RNA replication of MHV proceeds in a discontinuous and nonprocessive manner, thus generating free segmented RNA intermediates, which could be used in RNA recombination via a copy-choice mechanism.",
author = "Shinji Makino and Keck, {J. G.} and Stohlman, {S. A.} and Lai, {M. M C}",
year = "1986",
language = "English (US)",
volume = "57",
pages = "729--737",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - High-frequency RNA recombination of murine coronaviruses

AU - Makino, Shinji

AU - Keck, J. G.

AU - Stohlman, S. A.

AU - Lai, M. M C

PY - 1986

Y1 - 1986

N2 - The RNA genome of coronaviruses consists of a single species of nonsegmented RNA. In this communication, we demonstrate that the RNA genomes of different strains of murine coronaviruses recombine during mixed infection at a very high frequency. Susceptible cells were coinfected with a temperature-sensitive mutant of one strain of mouse hepatitis virus (MHV) and a wild-type virus of a different strain. Of 21 randomly isolated viruses released from the coinfected cells at the nonpermissive temperature, 2 were recombinants which differed in the site of recombination. After three serial passages of the original virus pool derived from the mixed infection, the majority of the progeny viruses were recombinants. These recombinant viruses represented at least five different recombination sites between the two parental MHV strains. Such a high-frequency recombination between nonsegmented RNA genomes of MHV suggests that segmented RNA intermediates might be generated during MHV replication. We propose that the RNA replication of MHV proceeds in a discontinuous and nonprocessive manner, thus generating free segmented RNA intermediates, which could be used in RNA recombination via a copy-choice mechanism.

AB - The RNA genome of coronaviruses consists of a single species of nonsegmented RNA. In this communication, we demonstrate that the RNA genomes of different strains of murine coronaviruses recombine during mixed infection at a very high frequency. Susceptible cells were coinfected with a temperature-sensitive mutant of one strain of mouse hepatitis virus (MHV) and a wild-type virus of a different strain. Of 21 randomly isolated viruses released from the coinfected cells at the nonpermissive temperature, 2 were recombinants which differed in the site of recombination. After three serial passages of the original virus pool derived from the mixed infection, the majority of the progeny viruses were recombinants. These recombinant viruses represented at least five different recombination sites between the two parental MHV strains. Such a high-frequency recombination between nonsegmented RNA genomes of MHV suggests that segmented RNA intermediates might be generated during MHV replication. We propose that the RNA replication of MHV proceeds in a discontinuous and nonprocessive manner, thus generating free segmented RNA intermediates, which could be used in RNA recombination via a copy-choice mechanism.

UR - http://www.scopus.com/inward/record.url?scp=0022633650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022633650&partnerID=8YFLogxK

M3 - Article

VL - 57

SP - 729

EP - 737

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 3

ER -