High Levels of Orexin A in the Brain of the Mouse Model for Phenylketonuria

Possible Role of Orexin A in Hyperactivity Seen in Children with PKU

Sankar Surendran, Gerald Campbell, Stephen K. Tyring, Kimberlee Matalon, J. David McDonald, Reuben Matalon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Phenylketonuria (PKU) is a metabolic disorder caused by phenylalanine hydroxylase deficiency leading to increased levels of phenylalanine in the brain. Hyperactivity is reportedly induced by a high level of orexin A, and therefore orexin A content was studied in the PKU mice. Hypothalamus and brain stem had higher levels of orexin A compared to cerebrum and cerebellum both in wild type and PKU mice brains as observed by radioimmunoassay method. Interestingly, all these regions of the brain in PKU mouse showed a higher level of orexin A compared to the wild type. Heart and plasma also had higher levels of orexin A in PKU compared to the wild type. Immunohistochemical analysis revealed an increased number of orexin A-stained cells in the brain and heart of PKU mouse compared to the wild type. This is the first report of increased level of orexin in the PKU mouse brain. Hyperactivity is commonly observed in children with PKU; thus these findings suggest that orexin A is a contributing factor for the hyperactivity.

Original languageEnglish (US)
Pages (from-to)1891-1894
Number of pages4
JournalNeurochemical Research
Volume28
Issue number12
DOIs
StatePublished - Dec 2003

Fingerprint

Phenylketonurias
Brain
Phenylalanine Hydroxylase
Orexins
Phenylalanine
Cerebrum
Cerebellum
Hypothalamus
Brain Stem
Radioimmunoassay
Plasmas

Keywords

  • Hyperactivity
  • Hypocretin 1
  • Mouse brain
  • Orexin A
  • Phenylketonuria

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

High Levels of Orexin A in the Brain of the Mouse Model for Phenylketonuria : Possible Role of Orexin A in Hyperactivity Seen in Children with PKU. / Surendran, Sankar; Campbell, Gerald; Tyring, Stephen K.; Matalon, Kimberlee; McDonald, J. David; Matalon, Reuben.

In: Neurochemical Research, Vol. 28, No. 12, 12.2003, p. 1891-1894.

Research output: Contribution to journalArticle

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