High molecular weight forms of insulin-like growth factor II and its binding protein identified by protein immunoblotting

Phillip Lee, D. R. Powell, C. H. Li, H. Bohn, F. Liu, R. L. Hintz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Insulin-like growth factor (IGF)II is a mitogenic polypeptide which circulates in association with a binding proteins(s). Immunoblotting studies were performed in human serum and indicate that:(1)a ∼200 kDa covalently-linked IGF-II/binding protein complex in antigenically related to the 30 kDa binding protein, (2)IGF-II prohormone is associated with this complex, and (3)a major portion of the IGF-II prohormone immunoreactivity in human serum is present in fractions which would not be detected by standard radioimmunoassay methods. Our data provide insight regarding the inter-relationships of IGF-II and its binding protein, and direct evidence for the presence of IGF-II prohormone in human serum.

Original languageEnglish
Pages (from-to)1131-1137
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume152
Issue number3
DOIs
StatePublished - May 16 1988
Externally publishedYes

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Insulin-Like Growth Factor II
Immunoblotting
Carrier Proteins
Molecular Weight
Molecular weight
Proteins
Serum
Insulin-Like Growth Factor Binding Proteins
Radioimmunoassay
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

High molecular weight forms of insulin-like growth factor II and its binding protein identified by protein immunoblotting. / Lee, Phillip; Powell, D. R.; Li, C. H.; Bohn, H.; Liu, F.; Hintz, R. L.

In: Biochemical and Biophysical Research Communications, Vol. 152, No. 3, 16.05.1988, p. 1131-1137.

Research output: Contribution to journalArticle

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