Insulin-like growth factor (IGF)II is a mitogenic polypeptide which circulates in association with a binding proteins(s). Immunoblotting studies were performed in human serum and indicate that:(1)a ∼200 kDa covalently-linked IGF-II/binding protein complex in antigenically related to the 30 kDa binding protein, (2)IGF-II prohormone is associated with this complex, and (3)a major portion of the IGF-II prohormone immunoreactivity in human serum is present in fractions which would not be detected by standard radioimmunoassay methods. Our data provide insight regarding the inter-relationships of IGF-II and its binding protein, and direct evidence for the presence of IGF-II prohormone in human serum.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 16 1988|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology