High molecular weight forms of insulin-like growth factor II and its binding protein identified by protein immunoblotting

Phillip Lee, D. R. Powell, C. H. Li, H. Bohn, F. Liu, R. L. Hintz

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Abstract

Insulin-like growth factor (IGF)II is a mitogenic polypeptide which circulates in association with a binding proteins(s). Immunoblotting studies were performed in human serum and indicate that:(1)a ∼200 kDa covalently-linked IGF-II/binding protein complex in antigenically related to the 30 kDa binding protein, (2)IGF-II prohormone is associated with this complex, and (3)a major portion of the IGF-II prohormone immunoreactivity in human serum is present in fractions which would not be detected by standard radioimmunoassay methods. Our data provide insight regarding the inter-relationships of IGF-II and its binding protein, and direct evidence for the presence of IGF-II prohormone in human serum.

Original languageEnglish
Pages (from-to)1131-1137
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume152
Issue number3
DOIs
StatePublished - May 16 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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