High performance liquid chromatographic method for the determination and pharmacokinetic studies of oxyresveratrol and resveratrol in rat plasma after oral administration of Smilax china extract

Huilian Huang, Jinqiang Zhang, Guangtong Chen, Zhiqiang Lu, Xiao-Ming Wang, Na Sha, Bo Shao, Ping Li, De An Guo

Research output: Contribution to journalArticle

30 Scopus citations


A sensitive and simple HPLC method has been developed and validated for the determination of oxyresveratrol (trans-2,4,3′,5′-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4′-trihydroxystilbene, RES) in rat plasma. The plasma samples were extracted with ethyl acetate and analyzed using HPLC on an Aglient Zorbax SB-C18 column (250 × 4.6 mm, 5 μm) at a wavelength 320 nm, with a linear gradient of (A) acetonitrile and (B) 0.5% aqueous acetic acid (v/v), at a flow rate of 1.0 mL/min. The method was linear over the range of 0.1265-25.3 μg/mL for OXY and 0.117-23.4 μg/mL for RES. The extraction recovery for OXY, RES and internal standard ranged from 71.1 to 88.3%. The intra- and inter-day precisions were better than 10%, and the accuracy ranged from 89 to 108%. The validated method was used to study the pharmacokinetic profiles of OXY and RES in rat plasma after oral administration of Smilax china root extract.

Original languageEnglish (US)
Pages (from-to)421-427
Number of pages7
JournalBiomedical Chromatography
Issue number4
StatePublished - Apr 2008
Externally publishedYes



  • HPLC
  • Oxyresveratrol
  • Pharmacokinetics
  • Resveratrol
  • Smilax china

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

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