A sensitive and simple HPLC method has been developed and validated for the determination of oxyresveratrol (trans-2,4,3′,5′-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4′-trihydroxystilbene, RES) in rat plasma. The plasma samples were extracted with ethyl acetate and analyzed using HPLC on an Aglient Zorbax SB-C18 column (250 × 4.6 mm, 5 μm) at a wavelength 320 nm, with a linear gradient of (A) acetonitrile and (B) 0.5% aqueous acetic acid (v/v), at a flow rate of 1.0 mL/min. The method was linear over the range of 0.1265-25.3 μg/mL for OXY and 0.117-23.4 μg/mL for RES. The extraction recovery for OXY, RES and internal standard ranged from 71.1 to 88.3%. The intra- and inter-day precisions were better than 10%, and the accuracy ranged from 89 to 108%. The validated method was used to study the pharmacokinetic profiles of OXY and RES in rat plasma after oral administration of Smilax china root extract.
- Smilax china
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)