High-Performance Liquid Chromatography Method for the Determination of Protein-Glutathione Mixed Disulfide

K. Kumari, P. Khanna, Naseem Ansari, Satish Srivastava

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have developed an efficient and sensitive method for the quantification of picomole quantities of protein-glutathione and protein-cysteine mixed disulfides in ocular lens. Performic acid oxidation releases glutathione and cysteine from the protein-mixed disulfides as glutathione sulfonic acid and cysteic acid, respectively. The sulfonic acids are derivatized with phenylisothiocyanate, and the phenylthiocarbamyl derivatives are separated by HPLC using a reversed-phase ODS column, equilibrated with 0.14 M sodium acetate, containing 0.1% triethylamine and 6% acetonitrile.

Original languageEnglish (US)
Pages (from-to)374-376
Number of pages3
JournalAnalytical Biochemistry
Volume220
Issue number2
DOIs
StatePublished - Aug 1 1994

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Glutathione Disulfide
High performance liquid chromatography
Disulfides
Glutathione
Sulfonic Acids
High Pressure Liquid Chromatography
Cysteine
Cysteic Acid
Sodium Acetate
Crystalline Lens
Proteins
Lenses
Derivatives
Oxidation
cysteine-glutathione disulfide

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

High-Performance Liquid Chromatography Method for the Determination of Protein-Glutathione Mixed Disulfide. / Kumari, K.; Khanna, P.; Ansari, Naseem; Srivastava, Satish.

In: Analytical Biochemistry, Vol. 220, No. 2, 01.08.1994, p. 374-376.

Research output: Contribution to journalArticle

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